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当前位置: 首页 > 文章 > 两种入侵粉虱热激基因Hsp70的克隆及温度胁迫下的种间差异表达 生态学杂志 2010,29 (4) 693-701
Position: Home > Articles > Cloning and inter-specific altered expression of heat shock gene Hsp70 in two invasive whiteflies in response to thermal stress Chinese Journal of Ecology 2010,29 (4) 693-701

两种入侵粉虱热激基因Hsp70的克隆及温度胁迫下的种间差异表达

作  者：
王海鸿;雷仲仁
单  位：
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
关键词：
B型烟粉虱;温室白粉虱;Hsp70基因;克隆;表达;温度胁迫;实时定量PCR
摘  要：
为了检测B型烟粉虱和温室白粉虱在受到温度胁迫时热激基因Hsp70的差异表达谱,克隆了2种粉虱Hsp70基因的全长cDNA序列,并用实时定量PCR的方法分析了在不同温度条件下该基因的表达谱。结果表明:B型烟粉虱和温室白粉虱的Hsp70基因BTH-sp70和TVHsp70(GenBank登录号分别为DQ093385和EU861391),在5’-非编码区(UTR)中有类似TATA-box样元件;在3’-UTR中有poly(A)信号AATAA和富含A-T区;根据基因开放阅读框序列(ORF)推导的氨基酸序列均含有全部3个Hsp70蛋白家族高度保守的基序。在整个检测的温度范围内(-19℃～46℃),诱导温室白粉虱Hsp70表达的起始温度(Ton)或最大温度(Tmax)要比B型烟粉虱低2.5℃～7.5℃。这些结果表明,所克隆的基因属于有功能的温度诱导型Hsp70基因;在基因表达水平上,温室白粉虱比B型烟粉虱更耐冷,而后者更耐热;Hsp70的Ton(或者Tmax)能代表这2种粉虱的温度耐受能力。本研究结果在一定程度上解释了自然界中这2种粉虱种群地理分布和季节发生差异的原因。
译  名：
Cloning and inter-specific altered expression of heat shock gene Hsp70 in two invasive whiteflies in response to thermal stress
作  者：
WANG Hai-hong,LEI Zhong-ren (State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词：
Bemisia tabaci B-biotype;Trialeurodes vaporariorum;Hsp70 gene;cloning;expression;temperature stress;real-time quantitative PCR
摘  要：
To investigate whether the heat shock gene 70 (Hsp70) in Bemisia tabaci B-biotype and Trialeurodes vaporariorum has different expression profile in response to temperature stress,the full length cDNA of the Hsp70 in the two whitefly species was cloned,and the expression profile of the Hsp70 across a temperature gradient was analyzed by real-time quantitative PCR.The Hsp70 gene of both B.tabaci B-biotype and T.vaporariorum,named BTHsp70 and TVHsp70 (GenBank accession nos:DQ093385 and EU861391,respectively),had TATA-box-like elements in 5'-UTR,poly(A) signals AATAA in 3'-UTR,and three highly conserved functional motifs of the Hsp70 protein family in deduced amino acid sequences.Across the temperature gradient (from-19 to 46 ℃),the onset temperature (Ton) or maximal temperature (Tmax) for inducing Hsp70 expression in T.vaporariorum was 2.5 ℃-7.5 ℃ lower than those for the expression in B.tabaci B-biotype.All the results revealed that the cloned genes were of inducible Hsp70 gene,and,in terms of gene expression level,T.vaporariorum was more cold-tolerant than B.tabaci B-biotype,while the latter was more heat-tolerant.The Ton (or Tmax) of the Hsps could represent the differences in temperature tolerance of these two whitefly species.To some extent,our results explained the different geographical distribution and seasonal occurrence of the two whitefly populations in nature.

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