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当前位置: 首页 > 文章 > 四株解磷菌分泌有机酸与溶解磷酸三钙能力的研究 新疆农业科学 2017 (6) 1114-1121
Position: Home > Articles > Study on Organic Acid Secreted from 4 Strains Phosphorus-solubilizing Bacteria and Calcium Phosphate Dissolving Ability Xinjiang Agricultural Sciences 2017 (6) 1114-1121

四株解磷菌分泌有机酸与溶解磷酸三钙能力的研究

作  者：
刘胜亮;朱舒亮;祁先慧;李晓原;张富玮;杨文英;李建贵
单  位：
新疆农业大学/新疆红枣工程技术研究中心;新疆农业大学2612团队;新疆农业大学林业研究所
关键词：
解磷微生物;酸解作用;有机酸
摘  要：
【目的】研究解磷细菌溶解难溶性磷酸盐及溶磷过程中有机酸的作用。【方法】对P7、P13、P15、P18四株解磷菌进行培养,并对其进行动态观测,用高效液相色谱法测定其有机酸种类及含量,研究其解磷量与有机酸之间的关系。【结果】4株解磷菌溶解磷酸三钙能力有显著差异,解磷能力大小依次是P15>P13>P18>P7。4株解磷菌产生有机酸的含量差异很大,并且同一株解磷菌在培养过程中产生的有机酸种类也会不同。经分析发现有机酸对速效磷的直接影响由强至弱排序依次是:丙酮酸、酒石酸、乳酸、乙酸、草酸。【结论】解磷能力最强的是P15菌株,丙酮酸对溶解磷酸三钙的直接影响系数最大。
译  名：
Study on Organic Acid Secreted from 4 Strains Phosphorus-solubilizing Bacteria and Calcium Phosphate Dissolving Ability
作  者：
LIU Sheng-liang;ZHU Shu-liang;QI Xian-hui;LI Xiao-yuan;ZHANG Fu-wei;YANG Wen-ying;LI Jian-gui;Forestry Institute,Xinjiang Agricultural University;Xinjiang Jujube Engineering Technology Research Center,Xinjiang Agricultural University;Xinjiang Agricultural University 2612 team;
关键词：
phosphate dissolving microorganism;;acid hydrolysis;;organic acid
摘  要：
【Objective】To study organic acid secreted from phosphorus-solubilizing bacteria and calcium phosphate dissolving ability.【Method】The P7,P13,P15 and P18 phosphate-solubilizing bacteria were sent to cultivate for dynamic observation. The organic acid types and content were determined by high performance liquid chromatography(HPLC) method for studying the relationship between phosphate-solubilizing and organic acids. 【Result】The research showed that dissolving calcium phosphate capacity of the 4 strains of bacteria had significant difference,the order of which was: P15 > P13 > P18 > P7 and the content difference of four kinds of bacteria producing organic acids was also great,and organic acids produced by the same bacteria at different times were different,too. Through the path analysis,it was found that the direct effects of organic acids on available phosphorus in descending order were: pyruvic acid > tartaric acid > lactic acid >acetic acid > oxalic acid.【Conclusion】The strongest ability to solve phosphorus bacteria is the P15 strain and the direct influence coefficient of pyruvic acid on the dissolution of calcium phosphate is the largest.

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