当前位置: 首页 > 文章 > 拮抗放线菌WS-03030的分类鉴定及其抗菌成分的初步研究 湖北农业科学 2008,47 (4) 68-70
Position: Home > Articles > Classification of Actinomycetes WS-03030 and Its Antifungal Substances Hubei Agricultural Sciences 2008,47 (4) 68-70

拮抗放线菌WS-03030的分类鉴定及其抗菌成分的初步研究

作  者:
陈涛风;王开梅;万中义;杨自文
单  位:
湖北省生物农药工程研究中心;中国科学院武汉病毒所
关键词:
农用抗生素;分类鉴定;分离纯化;抑菌活性
摘  要:
WS-03030是从武汉地区土壤中分离到的1株具有抗真菌活性的菌株。经形态学、培养特征、生理生化特征培养观察及16SrDNA序列分析,将WS-03030鉴定为吸水链霉菌;WS-03030发酵液的乙酸乙酯粗提物经半制备高效液相及活性测定确认了产生的活性物质,活性物质通过高效液相制得的纯品对稻瘟病菌、黄色镰刀菌和小麦颖枯病菌的最低抑制浓度(MIC)仅1.562 5μg.mL-1。
译  名:
Classification of Actinomycetes WS-03030 and Its Antifungal Substances
作  者:
CHEN Tao-feng1,2,WANG Kai-mei2,3,WAN Zhong-yi2,3,YANG Zi-wen2,3(1.Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430061,China;2.Hubei Biopesticide Engineering Research Center,Wuhan 430064,China;3.The Branch Center of Biopesiticides,Hubei Agricultural Science Innovation Center,Wuhan 430064,China)
关键词:
agro-antibiotics;classification;separation and purification;antifungal activity
摘  要:
In screening process of new agricultural antibiotics,a actinomycetes strain WS-03030 was isolated and identificated from a soil sample collected in Wuhan.The results showed that WS-03030 belonged to Streptomyces hygroscopicus.Its bioactive substance was isolated by ethyl acetate extraction and purified by preparative HPLC through activity-guiding method.Antifungal experiment showed that the substance' MIC to Pyricularia oryzae,Fusarium culmorum,Septoria nodorum could reach 1.562 5 μg·mL-1.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊