Association analysis between the polymorphism of the lectin- like ox- LDL receptor 1( LOX- 1) gene and milk yield in Jersey cattle

HUO Qiu-hong;TANG Yi-bo;JIANG Shi-qiang;YANG Fang;JIANG Ming-sheng;College of Animal Science and Technology,Guangxi University;

lectin-like ox-LDL receptor 1(LOX-1) gene;;Jersey cattle;;PCR;;SNPs;;milk yield

To study the correlation between the polymorphism of the lectin- like ox- LDL receptor 1( LOX- 1) gene and milk yield of Jersey cattle,the PCR product direct sequencing technique was used to analyze the polymorphisms of intron4 and 3'UTR of the LOX- 1 gene in Jersey cattle. The average 305- d milk yields of Jersey cattle with different genotypes were counted,and then the relevance between the different genotypes of the LOX- 1 gene with milk yield of Jersey cattle was analyzed. The results showed that two single nucleotide polymorphisms( SNPs)were detected in the intron4 of the LOX- 1 gene,and there were three genotypes in each SNP,namely CC,CT,TT and AA,AG,GG,respectively; but there was no significant differences( P > 0. 05) on the average 305- d milk yield of Jersey cattle between different genotypes.One SNP was detected in the 3'UTR of the LOX- 1 gene,and there were two genotypes in the SNP,namely CC and AC; in which,the genotype CC was dominant with genotypic frequency of 0. 909 1,and the average 305- d milk yield of Jersey cattle with CC genotype was also higher than that of Jersey cattle with AA genotype. It was found that the genotype combination CTAGCC was dominant through genotypes combination analysis,but there was no significant differences( P > 0. 05) on the average 305- d milk yield between different genotypes combinations.