中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 酪蛋白糖巨肽对小鼠肠道免疫系统的影响 食品科学 2010,31 (15) 240-243
Position: Home > Articles > Effect of Casein-glycomacropeptide on Intestinal Mucosal Immunity in Mice FOOD SCIENCE 2010,31 (15) 240-243

酪蛋白糖巨肽对小鼠肠道免疫系统的影响

作  者：
李伟;陈庆森
单  位：
天津市食品生物技术重点实验室;天津商业大学生物技术与食品科学学院
关键词：
酪蛋白糖巨肽;T淋巴细胞亚群;肠系膜淋巴结;PP结;脾脏
摘  要：
研究酪蛋白糖巨肽(CGMP)对正常小鼠肠相关淋巴组织的免疫应答反应的影响,对阐述CGMP的生物学功能具有重要意义。本实验将BALB/c雌性小鼠28只随机分为4组,每组7只,分别为0.9%生理盐水对照组(0.2mL/d),低、中、高3种剂量组(30、120、300μg/d)。连续灌胃16d后,取脾脏、PP结(peyers patches)和肠系膜淋巴结(mesenteric lymph node,MLN),制备细胞悬液,进行三色荧光标记后,用流式细胞仪检测CD3+、CD3+CD4+和CD3+CD8+T淋巴细胞亚群的变化。结果表明:CGMP各剂量组均能够促进脾脏和PP结中CD3+和CD3+CD4+细胞的显著增加(P<0.05),高剂量能引起脾脏CD3+CD8+淋巴细胞的显著增多(P<0.05),低剂量能引起脾脏CD3+CD4+/CD3+CD8+的显著增加(P<0.05);低剂量CGMP能使MLN中CD3+CD8+淋巴细胞显著增减少(P<0.01)。研究证实长期灌胃CGMP会诱导肠黏膜产生获得性的免疫应答,并且不会引起机体产生口服耐受。,
译  名：
Effect of Casein-glycomacropeptide on Intestinal Mucosal Immunity in Mice
作  者：
LI Wei,CHEN Qing-sen*(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)
关键词：
CGMP;T lymphocyte subgroup;MLN;peyer's patch;spleen
摘  要：
In order to explore the effect of casein-glycomacropeptide(CGMP) on the intestinal mucosal immune response in normal mice,28 female BALB/c mice were randomly divided into 4 groups for 7 mice in each group and by gavage given physiological saline(as control group) or CGMP at dosages of 30,120μg/d and 300μg/d(low-,medium-and high-dosage groups) for 16 consecutive days.After treatment,all the mice were sacrificed and their spleen,peyer's patches and mesenteric lymph node(MLN) were collected to examine the change of CD3+,CD3+CD4+ and CD3+CD8+T lymphocytes subpopulations using flow cytometry.The results indicated that CGMP resulted in a significant increase in CD3+ and CD3+CD4+ lymphocytes in spleen and peyer's patches(P < 0.05) in a dosage-dependent manner.Compared with the control group,high-dosage CGMP administration resulted in a higher percentage of CD3+CD8+ lymphocytes in spleen(P < 0.05),and low-dosage CGMP administration made the CD3+CD4+/CD3+CD8+ ratio significantly(P < 0.05) increase but the percentage of CD3+CD8+ lymphocytes in MLN significantly decrease(P<0.01).The above results demonstrate that long-term gastric perfusion of CGMP can induce intestinal mucosal adaptive immune response without tolerance to its oral administration.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46