作 者：
王桂芹;周洪琪;陈建明;赵朝阳;周辉;阎大伟;冷向军;叶金云;潘茜;王友慧
单 位：
上海水产大学生命科学与技术学院;浙江省淡水水产研究所
关键词：
翘嘴红鲌;饲料蛋白水平;大豆蛋白;增重率;蛋白质效率
摘 要：
选择健康的翘嘴红(Erythroculter ilishaeformisBleeker)为实验鱼,体质量(12.84±0.60)g。实验分为两部分进行。实验Ⅰ以褐鱼粉为蛋白源,配制5个蛋白水平(31.04%、35.51%、40.89%、46.62%、50.33%)的等能、等必需氨基酸(EAA)平衡关联度的半精制饲料,探讨翘嘴红对饲料蛋白的需求;经过8周饲养,实验Ⅰ的结果表明,饲料蛋白含量对翘嘴红的增重率、饲料效率和蛋白效率具有显著影响。方差分析表明,40.89%饲料蛋白组的鱼体增重率显著高于31.04%、35.51%饲料蛋白组(P<0.05),但是与46.62%和50.33%饲料蛋白组没有显著性差异(P>0.05);31.04%3、5.51%和40.89%饲料蛋白组的蛋白效率显著高于46.62%和50.33%饲料蛋白组(P<0.05);40.89%、46.62%和50.33%饲料蛋白组的饲料效率显著高于31.04%和35.51%饲料蛋白组(P<0.05)。依据折线模型分析翘嘴红的增重率、二次多项式回归分析蛋白质效率,适宜饲料蛋白水平为37.43%~41.15%。实验Ⅱ以豆粕替代鱼粉,大豆蛋白分别替代0.0%、13.5%、27%、40.5%和54%的鱼粉蛋白,配制5个EAA关联度的等蛋白(40%)、等能(20 MJ.kg-1)的半精制饲料,探讨翘嘴红饲料中大豆蛋白对鱼粉蛋白的适宜替代量。结果表明,当大豆蛋白分别替代13.5%2、7.0%和40.5%的鱼粉蛋白时,翘嘴红的增重率和蛋白效率与对照组差异不显著(P>0.05),而且都显著高于54.0%替代组(P<0.05),因此,本实验条件下,大豆蛋白对鱼粉蛋白的最大替代量为40.5%,饲料动、植物蛋白适宜比为3∶2。
译 名：
Nutrition requirement of dietary protein and optimal replacement of fish meal protein by soybean protein in Erythroculter ilishaeformis juveniles
作 者：
WANG Gui-qin~(1,2),ZHOU Hong-qi~(1),CHEN Jian-ming~(3),ZHAO Chao-yang~(1),ZHOU Hui~(1),YAN Da-wei~(1),LENG Xiang-jun~(1),YE Jin-yun~(3),PAN Qian~(3),WANG You-hui~(3)(1.College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090;2.ChinaCollege of Animal Science and Technology,Jilin Agriculture University,Changchun 130118,China;3.Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China)
关键词：
Erythroculter ilishaeformis Bleeker;dietary protein level;soybean protein ratio;weight gain;protein efficiency rate
摘 要：
The initial body weight of Erythroculter ilishaeformis was(12.84±0.60)g.In the first trial,in order to investigate the optimum dietary protein requirement of topmouth culter,Erythroculter ilishaeformis Bleeker juveniles,five groups of diets with isoenergetic(20MJ·g~(-1) gross energy) and same relativity of essential amino acid balance containing different protein levels(31.04%,35.51%,40.89%,46.62% and 50.33% DW) were prepared.In the second trial,five groups of diets with isonitrigenous(40% protein) and isoenergetic(20MJ·g~(-1) gross energy) and different relativity of essential amino acid balance with soybean protein replacing 0.0%,13.5%,27.0%,40.5% and 54% of fish meal protein,were formulated to determine optimal replacement level of soybean protein to fish meal protein.After 8-week feeding,The weight gain,feed efficiency,protein efficiency rate were significantly influenced by dietary protein levels(P<0.05).Weight gain of the fish fed on 40.89% protein diets were significantly higher than those of the fish fed on 31.04% and 35.51% protein diets respectively(P<0.01).But there were not significant difference from those in the fish fed on 46.62% and 50.33% protein diets(P>0.05).Based on broken-line and quadratic regression analysis of weight gain and protein efficiency rate,dietary protein level for juvenile Erythroculter ilishaeformis was 37.43%-41.15%.In the second experiment,when the replacement level for fish meal protein by soybean protein was 13.5%,27% and 40.5%,there were no significant difference in weight gain and protein efficiency ratio among the diets compared with control groups (P >0.05),and were much higher than weight gain in the fish fed on 54% replacement of soybean protein(P<0.05).It comes to the conclusion that 40.5% of fish meal protein can be replaced by soybean protein in the diet of Erythroculter ilishaeformis,and the animal-plant protein ratio was 3∶2.
