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Position: Home > Articles > Isolation and disinsection characteristic of Bacillus thuringiensis from redryed tobacco leaf in China Chinese Tobacco Science 2005,26 (1) 34-38

我国复烤烟叶苏云金杆菌的分离及杀虫特性测定

作  者:
高家合
单  位:
云南省烟草科学研究所
关键词:
复烤烟叶;苏云金杆菌;分离;杀虫特性
摘  要:
采用4%N aC l培养基选择分离法,从我国10个烟区采集的801个复烤烟样中,分离出苏云金芽孢杆菌736株。分离出的Bt菌经PC R毒素蛋白基因鉴定,其中含cryⅠ毒素蛋白基因的Bt菌有7株,出菌率为0.95%;含cryⅤ毒素蛋白基因的Bt菌21株,出菌率为2.85%;没有含cryⅢ毒素蛋白基因的Bt菌。对含毒蛋白基因cryⅠ或cryⅤ的28个Bt菌株用二龄烟草甲虫进行生物毒力测定,试验后9d有18个Bt菌株生物毒力测定校正死亡率均超过80%;试验后12d有9个Bt菌株生物毒力测定校正死亡率均超过95%。可见,用苏云金芽孢杆菌防治烟叶仓贮害虫是有潜力的。
译  名:
Isolation and disinsection characteristic of Bacillus thuringiensis from redryed tobacco leaf in China
作  者:
GAO Jia-he(Yunnan Tobacco Science Research Institute Yuxi 653100, China)
关键词:
redryed tobacco leaf Bacillus thuringiensis isolation disinsection characteristics
摘  要:
Bacillus thuringiensis strains were isolated from 801 tobacco sample collected from 10 tobacco production area in China by the method of 4%NaCl cutural medium.The identification results of PCR toxin gene indicated that 7 Bt isolates with plating efficiency of 0.95% contained cryⅠ toxin gene 21 Bt strains contained cryⅤ gene without Bt isolates containing cryⅢ gene. The bioassay toxicity of 28 Bt isolates containing cryⅠand cryⅤ gene was determinated to Lasioderma serricorneF. second instar. The results showed that the corrected mortality of 18 Bt exceeded 80% 9 days after inoculation and 9 Bt isolates exceeded 95% 12 days after inoculation. Bt is potential in pest control on stored leaf tobacco

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