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Position: Home > Articles > A method appropriate to extract the DNA of twin cured tobacco Forest By-Product and Speciality in China 2001 (3) 2-3

一种适于提取复烤烟叶DNA的方法

作  者:
任如意;魏继承;刘瑞林
单  位:
黑龙江省林副特产研究所;牡丹江师范学院生物系157012
关键词:
提取;复烤烟叶;DNA
摘  要:
以改良 CTAB法提取复烤烟叶 DNA,经 U- 3000紫外分光光度计测定,所获 DNA呈典型的吸收曲线,且 OD260/ OD280在 1. 7~ 1. 9之间;对烟草叶绿体不编码序列进行 PCR扩增,获得理想条带。
译  名:
A method appropriate to extract the DNA of twin cured tobacco
作  者:
REN Ru-yi WEI Ji-cheng LIU Rui-lin
关键词:
extraction;curl tobacco leaf;DNA
摘  要:
The DNA is extracted from curl tobacco leaf with modified CTAB method and make a typical absorption curve,which datum of OD260/OD280 is between 1.7- 1.9 tested by U- 3000 UV- spectrophotometer.The ideal band is obtained in subsequent polymerase chain reaction(PCR)aimed at the sequence of chloroplast non- coding protein

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