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当前位置: 首页 > 文章 > JM-3菌株抗菌物质发酵条件优化及对苹果腐烂病的防效 北方园艺 2018 (4) 54-59
Position: Home > Articles > Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma Northern Horticulture 2018 (4) 54-59

JM-3菌株抗菌物质发酵条件优化及对苹果腐烂病的防效

作  者：
张淑颖;曲田丽;赵川德
单  位：
青岛农业大学植物保护学院;青岛农业大学化学与药学院
关键词：
抗菌物质;发酵条件;防病作用;苹果腐烂病
摘  要：
以从剑麻中筛选出的1株对苹果腐烂病有较强拮抗作用的菌株JM-3为研究对象,采用单因子试验和正交实验对菌株JM-3的最适发酵培养基成分及发酵条件进行优化,并测定了其对苹果离体枝条腐烂病的防治效果,以期为该菌株的工业化生产提供参考依据。结果表明:菌株JM-3培养基各组分最佳配比为葡萄糖30g·L~(-1),蛋白胨20g·L~(-1),K_2HPO_4 1.0g·L~(-1),最佳发酵条件为初始pH 7,装液量150mL或100mL/250mL三角瓶,培养温度33℃,培养时间120h。在最佳发酵培养基和培养条件下,菌株JM-3抑菌能力提高32.4%。JM-3发酵液不能对苹果离体枝条腐烂病起到治疗作用,但能够显著预防腐烂病斑扩展,提前24~48h接种JM-3发酵液的预防保护效果最好,与对照农药苯醚甲环唑相比无显著差异。
译  名：
Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma
作  者：
ZHANG Shuying;QU Tianli;ZHAO Chuande;College of Plant Protection,Qingdao Agricultural University;College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University;
关键词：
antifungal substances;;fermentation condition;;disease protection;;Valsa ceratosperma
摘  要：
Strain JM-3 isolated from sisal was used as study object,the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions of JM-3,and the control effect on apple excised twigs against Valsa ceratosperma was tested,in order to provide reference for establishing industrial production.The results showed that optimum medium was glucose 30 g·L~(-1),peptone 20 g·L~(-1),K_2HPO_4 1.0 g·L~(-1).The optimal fermentation conditions were initial pH 7,medium capacity 150 mL or 100 mL/250 mL,temperature33 ℃,fermentation time 120 hours.Inhibitory activity of strain JM-3 fermentation solution increased32.4% under the optimum fermentation medium and culture conditions.The fermentative liquid of strain JM-3 could not cure canker lesion expansion,but it could prevent canker lesion expansion significantly.The preventive protection of strain JM-3 fermentative liquid was the best when it was inoculated on apple twigs before 24-48 hours,and its protective effect had no significant difference with difenoconazole.

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