作 者：
任晓慧;张永亮;刘松财;史秋梅;戴建威;胡魁
单 位：
河北科技师范学院动物科技系;吉林省五星动物保健药厂;吉林大学畜牧兽医学院
关键词：
生长激素释放肽-6;乳酸-乙醇酸共聚物;微球
摘 要：
以生物降解材料乳酸乙醇酸共聚物 (PL GA,L A- GA 85∶ 1 5 )为载体 ,采用复乳 -液中蒸发法制备含生长激素释放肽 - 6的乳酸乙醇酸共聚物微球 ,并通过体外药物释放试验评价载药微球的释药特征。结果得到收率、粒径大小和分布及含药量均满意的微球 ,其中包封率为 1 0 0 %,平均体积径为 3 .4 5μm ,收率为 79%。在 3 7℃、p H 7.0的生理等渗缓冲液中 ,首日释药 1 9.1 9%,其后以平均日释药 3 .2 3 %的速度释药 2 5 d。小鼠肌肉注射微球 3 0 d后 ,累积增重比注射生长激素释放肽 - 6、生理盐水分别高 2 2 .5 6 %(P<0 .0 5 )和 5 3 .1 7%(P<0 .0 1 )。结果表明 ,生长激素释放肽 - 6乳酸乙醇酸共聚物微球有望发展成为新型长效制剂
译 名：
Preparation of Poly(D,L-lactide-co-glycolide) Microspheres Containing Growth Hormone Release Peptide-6 and Effects on Animal Growth
作 者：
REN Xiao-hui~(1,2),ZHANG Yong-liang~(1*),LIU Song-cai~1,SHI Qiu-mei~3,DAI Jian-wei~1,HU Kui~3 (1.College of Animal Husbandry and Veterinary,Jilin University, Changchun 130062,China;2.College of Aqua- culture,Heibei Agricultural University,Qinhuangdao 066003, China;3.Faculty of Animal Science and Technology,Hebei Normal University of Science and Technology,Changli 066600,China;4.Five Star Health Protection Pharmaceutical Factory,Changchun 130062,China)
关键词：
growth hormone release peptide-6;poly(D,L-lactide-co-glycolide);microspheres
摘 要：
Sustained-release GHRP-6 microspheres were prepared by double emulsion-in liquid evaporation process,using biodegradable polylactic-co-glycolicacid as carrier and the drug release test of the microspheres was detected in vitro. The microspheres with good yield,suitable sized and satisfied drug loading was obtained,and significant GHRP-6 sustained was shown in the test of drug release in vitro. The entrapment efficiency was 100%,mean particle size 3.45 μm and the yield above 79%.In pH7.0 physiological isotonic buffer solution,after 19.19% of GHRP-6 was released first day,the microspheres released at rate of 3.23% per day for more than 25 d(r=0.877).After 30 days,accumulative increased body weights on the group injected with GHRP-6 microspheres was significantly higher 22.56%(P<0.05) and 53.71%(P<0.01) than those group injected with GHRP-6 and saline respectively;The conclusion is that the sustained-release GHRP-6 microspheres has been developed successfully as a new long acting GHRP-6 formulation.
