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无花果蛋白酶在PEG/(NH_4)_2SO_4双水相体系中的分配行为

作  者:
冯自立;马娜
单  位:
陕西理工学院陕西省资源生物重点实验室;陕西理工学院物理系
关键词:
无花果蛋白酶;双水相体系;分配系数K
摘  要:
双水相萃取(ATPS)是近年来发展起来的新型、廉价酶纯化方法。为了扩展该方法应用领域,对PEG(polyethylene glycol)/(NH4)2SO4双水相体系萃取无花果叶中无花果蛋白酶的实验条件进行研究,考察PEG相对分子质量、成相物质量分数、pH值、温度对无花果蛋白酶在两相体系中分配系数(K值)的影响。结果表明:当两相体系中PEG相对分子质量为1000、PEG质量分数为20%、(NH4)2SO4质量分数为19%、pH值为6、温度室温(25℃)时蛋白酶在两相体系中分配系数最高可达3.6。
译  名:
Partitional Behavior of Ficin in PEG/(NH_4)_2SO_4 Two-phase Aqueous System
作  者:
FENG Zi-li1,MA Na2 (1. Bio-resources Key Laboratory of Shaanxi Province,Shaanxi University of Technology,Hanzhong 723001,China; 2. Department of Physics,Shaanxi University of Technology,Hanzhong 723001,China)
关键词:
ficin;two-phase aqueous system;partition coefficient K
摘  要:
Aqueous two-phase system (ATPS) was a new and cheap purification method for ficin. The partition of Ficin in polyethylene glycol and ammonium sulfate aqueous two-phase systems was investigated. The effects of PEG molecular weight,polyethylene glycol concentration and ammonium sulfate concentration,pH and temperature on ficin partition coefficient were also explored. Results exhibited that ficin partition coefficient in two-phase aqueous system at the conditions of 20% PEG1000,19% (NH4)2SO4,pH 6 and temperature at 25 ℃ was 3.6.

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