中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 产甲壳素酶多粘类芽孢杆菌A1的筛选鉴定及发酵产酶条件研究 食品工业科技 2016,37 (13) 157-161
Position: Home > Articles > Screening of a strain of chitinase producing Paenibacillus polymyxa A1 and investigation on its fermentation conditions for chitinase production Science and Technology of Food Industry 2016,37 (13) 157-161

产甲壳素酶多粘类芽孢杆菌A1的筛选鉴定及发酵产酶条件研究

作  者：
杜敬河;王栩;朱永安;吴凤英;段杉
单  位：
华南农业大学食品学院;广东永昊食品有限公司
关键词：
甲壳素酶;多粘类芽孢杆菌;发酵条件
摘  要：
从土壤中分离得到一株产甲壳素酶的细菌,经形态学、生理生化及分子生物学鉴定,确定为多粘类芽孢杆菌,命名为A1。研究发现甲壳素、壳聚糖和淀粉可诱导该菌产甲壳素酶;添加尿素可提高甲壳素酶产量。本文研究确定该菌发酵产酶条件如下:培养基采用1%甲壳素(120目),0.05%Mg SO_4·7H_2O,1.2%尿素,0.5%Na Cl,0.07%K_2HPO_4,0.03%KH_2PO_4,0.3%酵母提取粉,初始p H4.5,接种龄12 h,接种量1%,于37℃培养78 h,在此条件下,该菌产甲壳素酶水平达到0.549 U/m L。该菌在对数期,其产酶曲线的上升略滞后于生长曲线变化;而在衰亡期,产酶曲线的降低略早于生长曲线的降低,说明该酶的mRNA的稳定性较差。
译  名：
Screening of a strain of chitinase producing Paenibacillus polymyxa A1 and investigation on its fermentation conditions for chitinase production
作  者：
DU Jing- he;WANG Xu;ZHU Yong-an;WU Feng-ying;DUAN Shan;College of Food Science,South China Agricultural University;Guangdong Engineering Research Center of Natural Active Substance;Guangdong Yonghao Foods Limited;
关键词：
Chitinase;;Paenibacillus polymyxa;;fermention condition
摘  要：
A strain of chitinase producing bacteria was isolated from soil,and was identified to be Paenibacillus polymyxa based on its morphological,physiological and biochemical characteristics and 16 S r DNA sequencing.The strain was named as A1.Chitin,chitosan and starch can induce A1 to produce chitinase.The adding of urea in medium improved the production of chitinase.The fermentation conditions for chitinase production were determined as follows: the fermentation medium consisting of 1% chitin( 120 mesh),0.05% Mg SO_4·7H_2O,1.2% CO( NH_2)_2,0.5% Na Cl,0.03% KH_2PO_4,0.07% K_2HPO_4,0.3% yeast extract powder,p H4.5,starter culture age 12 h,inoculum size 1%,fermenting at 37 ℃ for 78 h. Under such conditions,the chitinase activity achieved 0.549 U / m L. At the exponential phase,the rising of chitinase production curve slightly lagged behind the growth curve of A1,at the decline phase,the falling of chitinase production curve was slightly earlier than the falling of growth curve. The results indicated that the mRNA of the chitinase was unstable.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46