当前位置: 首页 > 文章 > 真菌诱导子对黄芪愈伤组织生长和代谢的影响 吉林农业大学学报 2011,33 (3) 293-300
Position: Home > Articles > Effects of Fungal Elicitors on Growth and Metabolism in Callus of Astragalus membranaceus (Fisch) Bge. Journal of Jilin Agricultural University 2011,33 (3) 293-300

真菌诱导子对黄芪愈伤组织生长和代谢的影响

作  者:
杜研;王康宇;王义;孙春玉;蒋世翠;张美萍
单  位:
吉林农业大学生命科学学院
关键词:
黄芪;愈伤组织;真菌诱导子;皂苷;次生代谢
摘  要:
以黄芪愈伤组织为试验材料,研究了不同种类真菌及同种真菌的不同浓度、不同添加时间和作用时间对黄芪愈伤组织生长和皂苷含量的影响。用真菌提取物对黄芪愈伤组织进行诱导处理后,检测黄芪愈伤组织的生长量和皂苷含量以及代谢过程中POD、PAL、PPO、CAT的酶活性和NO、MDA、H2O2的含量。结果表明:80μg/mL的散子囊菌作为诱导子处理黄芪愈伤组织效果最佳,细胞的鲜重和皂苷含量分别达到12.387 g和11.508 4 mg/g,并且在培养的第28天加入,作用时间为7 d,此时皂苷的产率达1.42%。真菌诱导子的加入可以激发黄芪细胞的防御性应答反应,产生氧迸发和一氧化氮迸发,细胞的氧化还原态势发生了明显的变化,膜脂过氧化产物MDA的含量大幅度提高,POD、PPO、CAT的活力出现波动性的变化,并具有一定的时序性,同时与次生代谢产物积累有关的PAL活力也得到大幅度提高。
译  名:
Effects of Fungal Elicitors on Growth and Metabolism in Callus of Astragalus membranaceus (Fisch) Bge.
作  者:
DU Yan,WANG Kang-yu,WANG Yi,SUN Chun-yu,JIANG Shi-cui,ZHANG Mei-ping College of Life Science,Jilin Agricultural University,Changchun 130118,China
关键词:
Astragalus membranaceus(Fisch)Bge.;callus;fungal elicitor;saponin;secondary metabolism
摘  要:
Effects of different fungal elicitors and different concentration,addition time and action time on the growth and saponin content in callus of Astragalus membranaceus(Fisch)Bge were studied.Growth yield,saponin content and enzyme activity of peroxydase phenylalnine,ammonialyase,polyphenoloxidase and hydrogen peroxidase and content of nitrogen monoxidum,malonaldehyde and hydrogen peroxide after culture treated with salysalic acid were measured.Results: The best effect of fungal elicitor is Eurotium,the best concentration of which is 80 μg /mL.The fresh weight and dry weight reach 12.387 g and 11.508 4 mg/g,respectively.The addition time is the 28th day and the action time is 7 days.At that time,the yield of saponin content reaches 1.42%.The results show that fungal elicitors trigger oxygen and nitrogen monoxidum burst,changes of cell redox status,and fluctuation of the activity of redox enzymes with a sequence.The content of MDA increased while the activity of phenylalnine ammonialyase was promoted.

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