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Position: Home > Articles > Isolation and Purification of Soybean Agglutinin from Feed and Testing Its Biological Activity Feed Review 2012 (7) 1-3

饲料中大豆凝集素的分离纯化与生物活性测定

作  者:
王立红;周玉凤;张勇;张俊霞;董惠峰;刘鼎阔
单  位:
鼎正动物药业有限公司;武清区畜牧水产发展服务中心
关键词:
大豆凝集素;生物活性;饲料
摘  要:
采用不同纯化方法制备大豆凝集素,其产率略有不同,其中采用N-乙酰基-D-半乳糖胺-Epoxy-Sepharose6B法制备产率最高,且N-乙酰基-D-半乳糖胺对大豆凝集素的亲和力较高,所制备的大豆凝集素活性测定与标准品一致,试验结果表明,该法是目前制备大豆凝集素的最优方法。
译  名:
Isolation and Purification of Soybean Agglutinin from Feed and Testing Its Biological Activity
作  者:
WANG Lihong1, ZHOU Yufeng2, ZHANG Yong1, ZHANG Junxia1, DONG Huifeng1, LIU Dingkuo1* ( 1. S&E Anmail Pharmaceutical Co., Ltd., Tianjin 300402, China; 2. Wuqing District Livestock and Fisheries Development Services Center, Tianjin 301700, China)
关键词:
soybean agglutinin; biological activity; feed
摘  要:
Different purification methods to prepare soybean agglutinin and its yield were slightly different, preparation production rate was the highest, when used the N acetyl D-galactose amine-Epoxy-Sepharose 6B and the N acetyl D-galactose amine on soybean agglutinin affinity is higher, the activity between the preparation of soybean agglutinin and standard had no different. The test results showed that the method was the best method for the preparation of soybean agglutinin.

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