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Position: Home > Articles > Genetic Transformation of Gene(PSY) into Soybean Journal of Hebei North University(Natural Science Edition) 2005,21 (2)

PSY基因对大豆的遗传转化

作  者:
抗艳红;龚学臣;季静;王萍;王罡
单  位:
天津大学农业与生物工程学院;淮海工学院海洋学院;河北北方学院农业科学系
关键词:
基因枪;PSY基因;大豆;遗传转化
摘  要:
以3个大豆品种的体细胞胚为受体,以PSY为目的基因,应用基因枪法对大豆进行了遗传转化,经大豆体细胞胚的继代筛选培养、萌发培养、芽诱导培养、生根培养和潮霉素选择培养,获得了完整的抗性再生苗,经PCR和PCR-Southern分析鉴定,初步证明外源PSY基因已整合到大豆的基因组中.
译  名:
Genetic Transformation of Gene(PSY) into Soybean
作  者:
KANGYan-hong~1,GONGXue-chen~1,JIJing~2,WANGPing~3,WANGGang~2(1.Hebei North University,Zhangjiakou,Hebei 075000,China;2.Tianjin university,Tianjin,300072,China;3.Weihai Institute of Technelogy,Marine school,Lianyungang,222005,China)
关键词:
particle bombardment;PSYgene;Soybean;The Genetic Transformation
摘  要:
Taking somatic embryo of three varieties of soybean as acceptor,PSY gene of soybean was transformed by particle bombardment.Hygromycin resistant regeneration plants of soybean were obtained by somatic embryo propagating cultivation,bourgeoning cultivation,inducting bud and striking root cultivation and hygromycin screening in selective condition.PCR and PCR-Southern analysis from the transferred gene plants proved that PSY has been transferred into genome of soybean.

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