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Position: Home > Articles > Transformation of Agaricus bisporus by Particle Bombardment of Fruit Body Gill Tissue Acta Edulis Fungi 2011,18 (3) 9-11

基因枪法遗传转化双孢蘑菇菌褶

作  者:
曹育博;刘天翔;周巍巍;高玉千;戚元成;申进文;邱立友
单  位:
河南农业大学生命科学学院农业部农业微生物酶工程重点实验室
关键词:
双孢蘑菇;基因枪;遗传转化;潮霉素B抗性
摘  要:
以双孢蘑菇(Agaricus bisporus)2793为试材,采用基因枪法对其菌褶进行遗传转化,相对转化率为2.69%,转化子经PCR检测表明,外源潮霉素B抗性基因已经被转入到菌丝体中。
译  名:
Transformation of Agaricus bisporus by Particle Bombardment of Fruit Body Gill Tissue
作  者:
CAO Yubo,LIU Tianxiang,ZHOU Weiwei,GAO Yuqian,QI Yuancheng,SHEN Jinwen,QIU Liyou College of Life Sciences,Henan Agricultural University,Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture,Zhengzhou,Henan 450002,China
关键词:
Agaricus bisporus;particle bombardment;genetic transformation;hygromycin B resistance
摘  要:
Transformation of Agaricus bisporus by particle bombardment of fruit body gill tissue was confirmed using the hygromycin B gene as a selective marker.PCR amplification revealed that DNA containing the resistance marker had been inserted into fungal hyphae with a transformation efficiency of 2.69%.

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