当前位置: 首页 > 文章 > 不同消毒方式对设施草莓花药愈伤组织诱导的影响 湖北农业科学 2017,56 (7) 1364-1365+1377
Position: Home > Articles > Effects of Different Sterilization Methods on Protected Strawberry Anther Callus Induction Hubei Agricultural Sciences 2017,56 (7) 1364-1365+1377

不同消毒方式对设施草莓花药愈伤组织诱导的影响

作  者:
吴鹏飞;王丽娟;刘昭;切岩祥和
单  位:
静冈大学农学部;天津农学院园艺园林学院
关键词:
草莓(Fragaria ananassa Duch.);花药;消毒方式;愈伤组织
摘  要:
以栽培品种京藏香草莓(Fragaria ananassa Duch.)花药(单核靠边期)为材料,75%乙醇为对照,研究了5种不同组合的消毒方式对草莓花药愈伤组织的诱导影响。结果表明,处理T_1经过Tween-80消毒后,用75%乙醇进行消毒30 s可以有效控制花药污染,同时还能减少花药褐化率;其次是处理T_3,该处理虽能减少污染,但是褐化率略高于T_1;处理T_0(CK)、T_2均有不同程度的污染,处理T4褐化率最高。
译  名:
Effects of Different Sterilization Methods on Protected Strawberry Anther Callus Induction
作  者:
WU Peng-fei;WANG Li-juan;LIU Zhao;KIRRIIWA Yoshikazu;Department of Horticulture,Tianjin Agricultural College;Faculty of Agriculture,Shizuoka University;
关键词:
strawberry(Fragaria ananassa Duch.);;anther;;disinfection method;;callus
摘  要:
Using varieties of "Jingzangxiang" strawberry(Fragaria ananassa Duch.) anther(mononuclear side) as materials,75%alcohol as a reference,the five different combination ways of disinfection effect on strawberry anther callus induction were studied. The experimental results showed that the treatment T_1 after Tween-80 disinfection disinfected with 75% alcohol could effectively control the pollution of anther,at the same time it also reduced the browning rate of anther. T_3 could reduce pollution,but the browning rate is slightly higher than the T_1,T_0(CK) and T_2 have different degrees of pollution,T_4 browning was the most serious.

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