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Position: Home > Articles > Establishment and Application of the PCR Assay for Detection of GPV Jilin Animal Husbandry and Veterinary Medicine 2009,30 (5) 8-10

鹅细小病毒PCR检测方法的建立及应用

作  者:
邵洪泽;李琳;毛文智;辛英;姚新华
单  位:
吉林省兽医科学研究所
关键词:
鹅细小病毒;PCR;检测
摘  要:
根据鹅细小病毒VP3基因设计合成一对引物,结果特异性地扩增出与预期片段大小相符的430bp核苷酸片段,建立了用于检测鹅细小病毒的PCR方法。此方法检测鹅细小病毒结果与病毒分离培养、电镜检查结果一致。通过特异性、敏感性及临床应用实验证明,每5L样品中获得阳性扩增结果中最小检出量为2.5×10-1.58ELD50;此方法用于临床检测鹅细小病毒是特异的、敏感的、可行的,本次实验建立的PCR方法使我省小鹅瘟的诊断,监测和防制工作上升到分子生物学水平。
译  名:
Establishment and Application of the PCR Assay for Detection of GPV
作  者:
SHAO Hong-ze,LI Lin,MAO Wen-zhi,XIN Ying,YAO Xin-hua(Veterinary Institute in Jilin Province,Changchun Jilin 130062)
关键词:
Goose Parvovirus virus(GPV);PCR;detection
摘  要:
According to Goose Parvovirus virus VP3 gene,primers were designed and synthesized.This method was shown to specifically amplify a 430 bp fragment from GPV.Therefore the established PCR technique provided a method for detection of the GPV.The result of the method for detection agree with other's,for example virus of isolating culture method,a method for detection in electron microscope.Test on a large number of clinical samples showed that the PCR method was highly specific and can be rapidly performed in a standard PCR setting.The PCR method can detect 2.5 × 10-1.58ELD50/5 L virus concentration.Test of clinical application showed that the PCR method was better than virus isolation method.Therefore the established PCR technique provided a sensitive,specific and reliable method for detection of the GPV

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