中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 鸭瘟病毒、鹅细小病毒和番鸭细小病毒三重PCR检测方法的建立 畜牧与兽医 2019 (7) 85-89
Position: Home > Articles > 鸭瘟病毒、鹅细小病毒和番鸭细小病毒三重PCR检测方法的建立 Animal Husbandry & Veterinary Medicine 2019 (7) 85-89

鸭瘟病毒、鹅细小病毒和番鸭细小病毒三重PCR检测方法的建立

作  者：
饶体宇;张益;鲜思美;包细明;李婷;吴伯梅;张友;吴专伟;彭庆波
单  位：
贵州大学动物科学学院
关键词：
鸭瘟病毒;鹅细小病毒;番鸭细小病毒;多重PCR
摘  要：
为建立鉴别鸭瘟病毒(DPV)、鹅细小病毒(GPV)和番鸭细小病毒(MDPV)的多重PCR检测方法,参考文献合成针对DPV UL6基因、GPV NS基因和MDPV VP1基因序列的特异性引物.通过优化多重PCR反应中的引物浓度和退火温度,建立快速鉴别3种病毒的多重PCR方法,并对该方法的特异性、敏感性和重复性进行检验.结果显示,该方法对禽流感病毒、新城疫病毒、鸭肝炎病毒、鸭源巴氏杆菌、鸭疫里氏杆菌的核酸扩增结果均为阴性,表明特异性较强;DPV、GPV和MDPV的最低核酸检出量分别为237.3 pg、22.45 pg和204.6 pg,表明敏感性良好;该方法对DPV+GPV+MDPV、DPV+GPV、DPV+MDPV、GPV+MDPV、DPV、GPV和MDPV的核酸均能扩增出与预期大小一致的目的条带,表明该多重PCR方法重复性较好.本研究方法具有特异性强、敏感性良好、重复性较好和快速简便等特点,可用于DPV、GPV和MDPV临床感染病例的联合检测与鉴别诊断.
作  者：
Rao Tiyu;Zhang Yi;Xian Simei;Bao Ximing;Li Ting;Wu Bomei;Zhang You;Wu Zhuanwei;Peng Qingbo;College of Animal Science, Guizhou University;Institute of Animal Disease Research of Guizhou Province;
关键词：
duck plague virus;;goose parvovirus;;Muscovy duck parvovirus;;multiplex PCR
摘  要：
To establish a multiplex PCR method for identification of duck plague virus, goose parvovirus and Muscovy duck parvovirus, specific primers were synthesized for the sequences of the UL6 gene of duck plague virus, the NS gene of goose parvovirus and the VP1 gene of Muscovy duck parvovirus. The primer concentration and annealing temperature were optimized in the multiplex PCR reaction, a multiplex PCR method was established for rapid identification of the three viruses, and the specificity, sensitivity and repeatability of the method were tested. The results showed that the nucleic acid amplified was negative for of the avian influenza virus, Newcastle disease virus, duck hepatitis virus, duck-origin Pasteurella and R. anatipestifer, indicating a high specificity of this multiplex PCR method. The minimum nucleic acid detection amounts of duck plague virus, goose parvovirus and Muscovy duck parvovirus were 237.3 pg, 22.45 pg and 204.6 pg, respectively, indicating a high sensitivity of this multiplex PCR method. This method could be used to amplify the target band consistent with the expected size of the nucleic acids of duck plague virus + goose parvovirus + Muscovy duck parvovirus, duck plague virus + goose parvovirus, duck plague virus + Muscovy duck parvovirus, goose parvovirus + Muscovy duck parvovirus, duck plague virus, goose parvovirus and Muscovy duck parvovirus. To sum up, the triple PCR was of high specificity, sensitivity and repeatability, and might be used for the combined detec-tion and differential diagnosis of clinical infection cases of duck distemper virus, goose parvovirus and Muscovy duck parvovirus.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46