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当前位置: 首页 > 文章 > 白洗猪线粒体DNA控制区分子遗传变异分析 中国畜牧杂志 2014,50 (5) 1-5
Position: Home > Articles > Genetic Variation Analysis of Mitochondrial DNA Control Region of Baixi Pigs Chinese Journal of Animal Science 2014,50 (5) 1-5

白洗猪线粒体DNA控制区分子遗传变异分析

作  者：
丁玫;韦雄;密国辉;龙威海;陈村年;陈祥
单  位：
贵阳市花溪区农业局;高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州省生猪健康养殖工程技术研究中心;安顺市畜牧兽医局
关键词：
线粒体DNA控制区;遗传多样性;种质资源保护;白洗猪
摘  要：
对濒临灭绝贵州地方猪种白洗猪线粒体DNA控制区进行分子遗传学研究,为其科学保种和合理开发利用提供科学依据。PCR扩增线粒体DNA控制区全序列,直接测序分析。结果表明:白洗猪线粒体DNA控制区全长1 254~1 314 bp,可以分为3个区;按中间重复区序列是否发生变异可以分为A、B 2种类型;聚类分析和单倍型遗传距离分析显示,各类型分布频率很不均衡,遗传多样性呈降低趋势。结果说明,贵州白洗猪可能有2个母系起源,该物种已经濒临灭绝,单倍型多样度较丰富,但是遗传多样性正在逐步丧失,亟需进行科学的保护。
译  名：
Genetic Variation Analysis of Mitochondrial DNA Control Region of Baixi Pigs
作  者：
DING Mei;WEI Xiong;MI Guo-hui;LONG Wei-hai;CHEN Chun-nian;CHEN Xiang;Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University;Huaxi Agriculture Bureau of Guiyang;Anshun Bureau of Animal Husbandry and Veterinary;Guizhou Engineering Technology Research Center for Healthy Pig Breeding;
关键词：
mitochondrial DNA control region;;genetic diversity;;germplasm resources;;baixi pigs
摘  要：
In order to provide a scientific basis on breed conservation and reasonable exploitation and utilization of the Baixi pig, an endangered indigenous pig breed in Guizhou, the mitochondrial DNA control region of it was studied with molecular genetic methods. The complete sequence of the mitochondrial DNA control region was amplified by PCR and analysis were carried out after sequencing. The result showed the complete sequence of the mitochondrial DNA control region is 1254~1314 bp, and it could be divided into three distinct regions. The Baixi pig could be divided into A and B type by judging whether there exist mutations in the repeated sequence area. The results of cluster analysis and haploid genetic distance analysis revealed that the distribution frequency of each type was very uneven and the genetic diversity showed a decreased tendency. In condasion there may be two maternal origins for the Baixi pigs of Guizhou, and the species is on the verge of extinction. Though the haplotype diversity is relatively rich, the genetic diversity of this species is gradually lost, and urgent scientific protection is needed.

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