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当前位置: 首页 > 文章 > 基于功能化多壁碳纳米管的乙酰胆碱酯酶生物传感器制备 食品科学 2012,33 (4) 282-286
Position: Home > Articles > Preparation of Acetylcholinesterase Biosensor Based on Functionalized Multi-walled Carbon Nanotubes for Pesticides Detection FOOD SCIENCE 2012,33 (4) 282-286

基于功能化多壁碳纳米管的乙酰胆碱酯酶生物传感器制备

作  者：
孙霞;赵文苹;刘中合;王相友
单  位：
山东理工大学农业工程与食品科学学院;中国农业大学工学院
关键词：
生物传感器;乙酰胆碱酯酶;有机磷农药残留;功能化多壁碳纳米管
摘  要：
将功能化多壁碳纳米管(FCNTs)结合壳聚糖(CHIT)和戊二醛(GTA)导电聚合物形成凝胶溶胶,将该凝胶溶胶悬滴在玻碳电极(GCE)表面制得FCNTs-CHIT-GTA/GCE电极,结合自制的乙酰胆碱酶膜生成一种反应灵敏、快速、稳定、用于检测有机磷农药的电流型生物传感器。结果表明:该传感器具有出峰电位低、峰流值高、电子传递速率高、阻抗值小等优点,在研究不同浓度敌敌畏对酶抑制率的关系时,得到的关系曲线线性良好,最低检出限为20ng/L。该传感器适用于有机磷农药残留跟踪检测。
译  名：
Preparation of Acetylcholinesterase Biosensor Based on Functionalized Multi-walled Carbon Nanotubes for Pesticides Detection
作  者：
SUN Xia1,ZHAO Wen-ping1,2,LIU Zhong-he1,WANG Xiang-you1, (1.School of Agricultural and Food Engineering,Shandong University of Technology,Zibo 255049,China; 2.College of Engineering,China Agricultural University,Beijing 100083,China)
关键词：
biosensor;acetylcholinesterase;pesticide residue;functionalized multi-walled carbon nanotubes
摘  要：
This paper describes the use of functionalized multiwalled carbon nanotubes(FCNTs) based conductive polymer for designing acetylcholinesterase(AChE) biosensor.Transducers were obtained by directly dropping the sol-gel solution formed from FCNTs,chitosan(CHIT) and glutaraldehyde(GTA) on the surface of glassy carbon electrode(GCE).Acetylcholinesterase(AChE) as bio-recognizer was immobilized onto a CHIT membrane to recognize pesticides selectively.Before the detection,the CHIT enzyme membrane was quickly fixed on the surface of FCNTs-CHIT-GTA/GCE with O-ring,and thus a sensitive,fast and stable amperometric sensor for quantitative determination of organophosphates(OP) was developed.The electrochemical behavior of AChE-FCNTs-CHIT-GTA/GCE was studied and the results showed the FCNTs-CHIT-GTA promoted electron-transfer reactions at a low potential and catalyzed the electro-oxidation of thiocholine,thus increasing detection sensitivity.Based on the inhibition of OP on AChE activity,using dichlorvos as a model pesticide,the biosensor had good linearity in the concentration range 25-50 ng/L and 50-100 μg/L with a correlation coefficient of 0.9995 and 0.9991,respectively.The detection limit was 20 ng/L.The biosensor exhibited high sensitivity and good reproducibility and stability,and it was suitable for trace detection of OP pesticide residue.

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