Optimization of Tissue Culture Conditions and Establishment of Regeneration System for Populus×euramericana cl.'J2'

ZHANG Lei1,SU Xiao-hua2*,ZHANG Bing-yu2,HUANG Qin-jun2,ZHANG Xiang-hua2,LI Yi-liang(1.Forestry College,Shenyang Agriculture University,Shenyang 110161,Liaoning,China;2.Research Institute of Forestry,CAF;Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China)

P.×euramericana cl.'J2';tissue culture;regeneration system

The key factors affecting the growth of Populus×euramericana cl.'J2' in vitro and the optimal tissue culture conditions for it were ascertained.The high frequency regeneration system for P.×euramericana cl.'J2' was also established in this study.The optimum basic culture medium was MS;the optimal subculture medium and root inducement culture medium for shoot were 1/2MS + NAA 0.08 mg·L-1 + IBA 0.08·mg L-1;active carbon disadvantaged the growth of P.×euramericana cl.'J2';photoperiod had little effect on P.×euramericana cl.'J2',while light intensity and the pH value of subculture medium had great effect on it,the optimal light intensity was 2 500 lx and the optimal pH value was 6.5;the optimal shoot inducement culture medium for leaf-explant was MS + BA1.0 mg·L-1+ NAA0.1 mg·L-1 and for leafstalk-explant was MS + BA 1.0 mg·L-1+ NAA 0.2 mg·L-1.