Position: Home > Articles > Effects of Vitreoscilla Hemoglobin Gene(vgb) Expression on the Capacity for Nitrogen Utilization in Populus×euramericanacl.'J2'
Acta Agriculturae Boreali-occidentalis Sinica
2013,22
(12)
164-169
透明颤菌血红蛋白基因vgb的表达对杨树氮素利用能力的影响
作 者：
刘浡洋;褚延广;丁昌俊;张伟溪;崔树俊;沈应柏;苏晓华
单 位：
林木遗传育种国家重点实验室;云南省红河州泸西县中枢镇农业机械管理服务站;北京林业大学生物科学与技术学院
关键词：
京2杨;透明颤菌血红蛋白基因;非损伤微测技术;氮素利用
摘 要：
以转透明颤菌血红蛋白基因(vgb)京2杨为材料,采用qRT-PCR分析方法确认其外源基因在转基因无性系中可以正常表达。盆栽试验结果显示,在正常生长条件下转基因无性系的株高和地径显著提高,同时叶片全氮质量分数上升。进而利用非损伤微测技术(Non-invasive Micro-test Technique,NMT)对植株根部NO3-吸收情况的检测发现,在含有0.5mmol/L NO3-测试液中转基因无性系NO3-吸收速率大于非转基因植株。此外,研究还发现,转基因无性系的硝酸还原酶(nitrate reductase,NR)活性显著提高。可见:vgb的表达能提高杨树的氮素利用能力。
译 名：
Effects of Vitreoscilla Hemoglobin Gene(vgb) Expression on the Capacity for Nitrogen Utilization in Populus×euramericanacl.'J2'
作 者：
LIU Boyang;CHU Yanguang;DING Changjun;ZHANG Weixi;CUI Shujun;SHEN Yingbai;SU Xiaohua;Key Laboratory of Genetics and Breeding in Forestry Trees Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University;State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry;Agricultural Machinery Management Service Station of Zhongshu Town,Luxi County;
关键词：
P.×euramericana cl.‘J2';;Vitreoscilla hemoglobingene(vgb);;Non-invasive microtest technique(NMT);;Nitrogen utilization
摘 要：
Transgenic Populus×euramericanacl.‘J2'with Vitreoscilla hemoglobin gene(vgb)was used in this study.QRT-PCR assays confirmed the expression of vgbin transgenic lines.Transgenic lines showed greater height and basal diameter than control plants under normal growth conditions.A higher content of total nitrogen was observed in transgenic lines.Non-invasive Micro-test Technique(NMT)was used to detect the nitrate absorb flux of root,and the results showed that the roots of transgenic plants have higher absorb flux than control in solution containing 0.5mmol/L NO3-.Moreover,compared with the control,an increased activity of nitrate reductase was detected in transgenic lines.These results suggested that the expression of vgb could increase the capacity for nitrogen utilization in poplar.
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