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Position: Home > Articles > An Universal Quantitative Detection Method for Genetically Modified Soybean Event MON89788 Using Duplex Digital PCR FOOD SCIENCE 2018 (4) 312-319

转基因大豆MON89788双重数字PCR通用定量检测方法的建立

作  者：
刘津;李婷;冼钰茵;吴希阳;凌莉;高东微
单  位：
暨南大学理工学院;广东检验检疫技术中心广东省动植物与食品进出口技术措施研究重点实验室
关键词：
微滴式数字PCR;芯片式数字PCR;转基因大豆MON89788品系;定量检测
摘  要：
建立一种特异、稳定、灵敏、通用的转基因大豆MON89788品系双重数字聚合酶链式反应(digital polymerase chain reaction,d PCR)定量检测方法,在一个体系内同时进行内外源基因的定量检测,适用于微滴式数字PCR(droplet digital PCR,dd PCR)和芯片式数字PCR(chip digital PCR,cd PCR)平台,并通过了食品分析能力评价体系国际能力验证项目的盲样检测评价。dd PCR平台对大豆MON89788品系和内源Lectin的绝对定量限分别为8.0 copies/μL和8.2 copies/μL;cd PCR平台对大豆MON89788品系和内源Lectin的绝对定量限分别为7.443 copies/μL和7.646 copies/μL;dd PCR和cd PCR对转基因大豆MON89788品系成分相对含量的相对定量限均为0.1%。
译  名：
An Universal Quantitative Detection Method for Genetically Modified Soybean Event MON89788 Using Duplex Digital PCR
作  者：
LIU Jin;LI Ting;XIAN Yuyin;WU Xiyang;LING Li;GAO Dongwei;Guangdong Key Laboratory of Import and Export Technical Measures of Animal,Plant and Food,Guangdong Inspection and Quarantine Technology Center;College of Science and Engineering, Jinan University;
关键词：
droplet digital PCR;;chip digital PCR;;GM soybean event MON89788;;quantitative detection
摘  要：
An universal quantitative detection method for genetically modified(GM) soybean event MON89788 using duplex digital PCR(d PCR) with satisfying specificity, stability and sensitivity was established in this paper. This method could quantify both the endogenous and exogenous genes in a single reaction system and was demonstrated to be suitable for both droplet digital PCR(dd PCR) and chip digital PCR(cd PCR) platforms. The method then was used to qualify blind samples by a food analysis performance assessment scheme(FAPAS) test. The absolute limits of quantitation(LOQs) of dd PCR for MON89788 event-specific gene and lectin endogenous gene were 8.0 copies/μL and 8.2 copies/μL, respectively, while those of cd PCR were 7.443 copies/μL and 7.646 copies/μL, respectively. The relative LOQs of MON89788 were both 0.1% by dd PCR and cd PCR.

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