作 者：
雒诚龙;邵伟;任万平;赵艳坤;王东海;余雄
单 位：
新疆农业大学动物科学学院;新疆肉乳用草食动物营养重点实验室
摘 要：
【目的】诱导3T3-L1细胞分化为3T3-L1脂肪细胞,研究不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响。【方法】利用三联诱导法将3T3-L1细胞经诱导分化为3T3-L1脂肪细胞。当3T3-L1脂肪细胞密集后分别采用0(对照组)、0.2、0.5、1μmol/L生物素处理,分别在12 h、24 h与48 h时检测细胞上清液中PK mRNA、GLUT-4 mRNA、FAS mRNA及ACC1 mRNA相对表达量。【结果】在试验12h时:各试验组GLUT-4、PK、ACC1 mRNA相对表达量均极显著(P<0.01)高于对照组,1μmol/L组与0.5μmol/L组FAS mRNA相对表达量极显著(P<0.01)高于对照组与0.2μmol/L组;在试验24 h时:1μmol/L组GLUT-4 mRNA与PK mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组ACC1 mRNA相对表达量极显著(P<0.01)高于对照组,0.2μmol/L组与0.5μmol/L组ACC1 mRNA相对表达量显著(P<0.05)高于对照组;在试验48 h时:1μmol/L组GLUT-4 mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组PK mRNA相对表达量极显著(P<0.01)高于对照组,0.5μmol/L组FAS mRNA相对表达量极显著(P<0.05)高于1μmol/L组,1μmol/L组FAS mRNA相对表达量显著(P<0.05)高于对照组,1μmol/L组ACC1mRNA相对表达量极显著(P<0.01)高于其它组。【结论】生物素的添加可以提升脂肪细胞GLUT-4、PK、FAS与ACC1 mRNA相对表达量,且当以1μmol/L浓度作用时对GLUT-4、PK与ACC1提升效果最佳,以0.5μmol/L浓度作用时对FAS提升效果最佳。
译 名：
The Impact of Various Biotin Levels on Adipogenetic Relevant Gene Transcription and Expression of 3T3-L1 Adipocytes
作 者：
LUO Cheng-long;SHAO Wei;REN Wan-ping;ZHAO Yan-kun;WANG Dong-hai;YU Xiong;Collage of Animal Science,Xinjiang Agricultural University;Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition;
关键词：
biotin;;3T3-L1 adipocytes;;adipogenesis
摘 要：
【Objective】In this study,the purpose is to induce 3 T3-L1 cells to differentiate into 3 T3-L1 adipocytes and to study the effects of various biotin levels on the transcription and expression of adipose synthase related genes in 3 T3-L1 adipocytes.【Method】3 T3-L1 cells were induced to differentiate into 3 T3-L1 adipocytes by triplex induction. When 3 T3-L1 adipocytes were dense,they were treated by 0 μmol/L biotin( control group),0. 2,0. 5 and 1 μmol/L The relative expression of competitive PKmRNA,GLUT-4 mRNA,FAS mRNA and ACC1 mRNA in cell supernatant was detected at 12 h,24 h and 48 h,respectively.【Result】Results showed that when the test time was 12 h: GLUT-4 mRNA,PK mRNA and ACC1 mRNA relative expression quantity of experimental groups was significantly( P < 0. 01) higher than that of control group,FAS mRNA relative expression quantities of 0. 5 μmol/L group and 1 μmol/L group were significantly( P < 0. 01) higher than that of control group. When the test time was 24 h: GLUT-4 mRNA and PK mRNA relative expression quantity of 1 μmol/L group was significantly( P < 0. 05) higher than that of control group,ACC1 mRNA relative expression quantity of 1 μmol/L group was extremely significantly higher( P < 0. 01)than that of control group,ACC1 mRNA relative expression quantity of 0. 2 μmol/L group and 0. 5 μmol/L group was significantly higher( P < 0. 05) than that of control group. When the test time was 48 h,GLUT-4 mRNA relative expression quantity of 1 μmol/L group was significantly( P < 0. 05) lower than that of control group,PK mRNA relative expression quantity of 1 μmol/L group was extremely significantly( P < 0. 01)higher than that of control group,FAS mRNA relative expression quantity of 0. 5 μmol/L group was significantly( P < 0. 05) higher than that of 1 μmol/L group,FAS mRNA relative expression quantity of 1 μmol/L group was significantly( P < 0. 05) higher than that of control group,ACC1 mRNA relative expression quantity of 1 μmol/L group was extremely significantly( P < 0. 01) higher than that of other groups.【Conclusion】The addition of biotin can inhibit the adipogenesis through promoting the relative expression of GLUT-4,PK,FAS and ACC1 mRNA in adipocytes. Moreover,when the concentration is equal to 1 μmol/L,the effect is optimal to GLUT-4,PK and ACC1 mRNA,and when the concentration is equal to 0. 5 μmol/L,the effect is optimal to FAS mRNA.
