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当前位置: 首页 > 文章 > 高效液相色谱-质谱法同时测定婴幼儿配方奶粉中叶酸、VB_(12)和生物素 食品科学 2013,34 (22) 269-272
Position: Home > Articles > Simultaneous Determination of Folic Acid,Vitamin B_(12) and Biotin in Infant Formula by HPLC-MS-MS FOOD SCIENCE 2013,34 (22) 269-272

高效液相色谱-质谱法同时测定婴幼儿配方奶粉中叶酸、VB_(12)和生物素

作  者：
王凤玲;刘爱国;孙佳佳;彭诚
单  位：
天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室;天津商业大学生物技术与食品科学学院
关键词：
叶酸;VB12;生物素;等电点沉淀;高效液相色谱-质谱
摘  要：
建立高效液相色谱-质谱法对婴幼儿配方奶粉中叶酸、VB12和生物素同时定性和定量分析方法。采用等电点沉淀(pH1.90/4.70)法提取样品,SB-Aq(2.1mm×100mm,1.8μm)色谱柱分离,0.1%(V/V)甲酸溶液和乙腈作流动相,等度(16.5:83.5,V/V)洗脱,质谱MRM检测。结果表明:叶酸、VB12和生物素在5min内得到很好的分离。线性范围分别为3.5~250ng/mL、0.8~5.0ng/mL、0.7~50ng/mL,加样回收率平均值分别为97.8%、102.3%、96.9%,RSD分别为1.33%、0.9%、1.37%。该方法具有较好的精密度和准确性、灵敏度高、操作简单快速,可以应用于婴幼儿配方奶粉中叶酸、VB12和生物素的含量同时测定。
译  名：
Simultaneous Determination of Folic Acid,Vitamin B_(12) and Biotin in Infant Formula by HPLC-MS-MS
作  者：
WANG Feng-ling;LIU Ai-guo;SUN Jia-jia;PENG Cheng;Tianjin Key Laboratory of Food and Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce;
关键词：
folic acid;;vitamin B12;;biotin;;high performance liquid chromatography tandem mass spectrometry(HPLC-MS-MS);;isoelectric precipitation
摘  要：
A method using high performance liquid chromatography tandem mass spectrometry(HPLC-MS-MS) for simultaneous determination of folic acid,vitamin B12 and biotin in infant milk power was presented.Samples were sequentially extracted and then subjected to isoelectric precipitation(pH 1.90/4.70).The analysis was carried out on an SB-Aq column(2.1 mm × 100 mm,1.8 μm) with isocratic elution using a mobile phase made up of 0.1%(V/V) aqueous formic acid and acetonitrile(16.5:83.5,V/V).The analytes were detected in the MRM mode.Folic acid,vitamin B12 and biotin in samples could be separately well within 5 min.The calibration curves were linear over the concentration range of 3.5- 250 ng/mL for folic acid,0.8- 5 ng/mL for vitamin B12,and 0.7- 50 ng/mL for biotin.The average recovery rates were 97.8%,102.3% and 96.9%,with RSD of 1.33%,0.9% and 1.37% for folic acid,vitamin B12 and biotin,respectively.The developed method is precise,accurate,sensitive,fast and simple,which can thereforebe applied for the analysis of folic acid,vitamin B12 and biotin in infant formula.

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