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Position: Home > Articles > Preparation and Identification of Bovine β-casein Polyclonal Antibody Animal Husbandry and Feed Science 2011,32 (1) 105-106+112

牛β-酪蛋白多克隆抗体的制备及鉴定

作  者:
Yi Ha
单  位:
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018 China
关键词:
casein;polyclonal antibody;purified;antibody;rabbit antibody;blo
摘  要:
以牛β-酪蛋白标准品为免疫原,免疫健康的新西兰大白兔,制备了兔抗牛β-酪蛋白的多克隆抗体。经亲和层析纯化后,用SDS-PAGE电泳及考马斯亮蓝染色检测抗体的纯度,经ELISA法测定β-酪蛋白抗体的效价为1∶1600。Western-blot分析发现,制备的抗体与β-酪蛋白可以发生特异性结合,表明成功制备了纯度较高和特异性较好的牛β-酪蛋白多克隆抗体,为乳腺生物反应器的研究提供了有效的工具。
译  名:
Preparation and Identification of Bovine β-casein Polyclonal Antibody
作  者:
YANG Miao1,HOU Xian-zhi1,YANG Yin-fen1,KAO Gui-lan1,GAO Ai-wu2,WANG Xiu-mei1,HAN Ya-nan1(1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China)
关键词:
β-casein;polyclonal antibody;ELISA;western-blot
摘  要:
Polyclonal antibody against β-casein was successfully prepared by using the purified β-casein as immunogen.The rabbit antibody was purified by protein G resin affinity chromatography.The ELISA titer of the antibody against β-casein was 1∶1 600.Western-blot analysis showed that the antibody could specifically bind with the β-casein.The rabbit antibody against β-casein might provide an effective tool for mammary gland bioreactor research.

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