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Position: Home > Articles > Preparation and identification of bovine β-casein polyclonal antibody Chinese Veterinary Science 2010 (11) 1180-1183

牛β-酪蛋白多克隆抗体的制备及鉴定

作  者:
Yi Ha
单  位:
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018 China
关键词:
casein;polyclonal antibody;purified;antibody;rabbit antibody;blo
摘  要:
以牛β-酪蛋白标准品为免疫原,免疫健康新西兰白兔,制备了兔抗牛β-酪蛋白的多克隆抗体。经亲和层析纯化后,用SDS-PAGE电泳及考马斯亮蓝染色检测抗体的纯度,经ELISA法测定β-酪蛋白抗体的效价为1∶1600,Western-blot分析发现制备的抗体与β-酪蛋白可以发生特异性结合。表明,成功制备了纯度较高和特异性较好的牛β-酪蛋白多克隆抗体,为乳腺生物反应器的研究提供了有效的工具。
译  名:
Preparation and identification of bovine β-casein polyclonal antibody
作  者:
YANG Miao a,HOU Xian-zhi a,YANG Yin-fen a,KAO Gui-lan a,GAO Ai-wu b,WANG Xiu-mei a,HAN Ya-nan a(a.College of Animal Science and Veterinary Medicine;b.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China)
关键词:
β-casein;polyclonal antibody;ELISA;Western-blot
摘  要:
Polyclonal antibody against β-casein was successfully prepared by using the purified β-casein as immunogen.The rabbit antibody was purified by protein G resin affinity chromatography.The ELISA titer of the antibody against β-casein was 1∶1600.Western-blot analysis showed that the antibody was able to specifically bind the β-casein.The rabbit antibody against β-casein might provide an effective tool for studying mammary gland bioreactor.

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