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当前位置: 首页 > 文章 > 甘肃现代肉羊新品种群羊GH基因外显子1多态性与体重关联分析 生物技术通报 2010,0 (12) 163-166
Position: Home > Articles > Polymorphism of Exon 1 of Growth Hormone Gene and Its Relationship with Body Weight of the Gansu Modern Meat Sheep New Breed Population Biotechnology Bulletin 2010,0 (12) 163-166

甘肃现代肉羊新品种群羊GH基因外显子1多态性与体重关联分析

作  者：
张浩;朱爱文;马友记;李发弟;陈国宏
单  位：
甘肃农业大学动物科学与技术学院
关键词：
生长激素基因;绵羊;PCR-SSCP;遗传多态性;体重
摘  要：
根据GenBank发表的绵羊生长激素(GH)基因外显子1的序列设计一对引物,采用PCR-SSCP技术分析GH基因外显子1在甘肃现代肉羊新品种选育群羊中的单核苷酸多态性,并与3月龄前的体重进行关联分析。结果表明,GH基因外显子1在新品种群羊中存在多态性,检测到两种基因型(AA、AB),其301bp处有一个T/A突变和305bp处有一个G/A突变,初生重、1月、2月、3月龄体重AA、AB型都无显著性差异(P>0.05),但3月龄AB型个体的体重相对于AA型偏高,由此初步推断GH基因可能是影响绵羊体重性状的主基因或与主基因相连锁,可用以对绵羊体重性状进行标记辅助选择。
译  名：
Polymorphism of Exon 1 of Growth Hormone Gene and Its Relationship with Body Weight of the Gansu Modern Meat Sheep New Breed Population
作  者：
Zhang Hao Zhu Aiwen Ma Youji Li Fadi Chen Guohong(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070)
关键词：
Growth hormone gene Sheep PCR-SSCP Genetic polymorphism Body weight
摘  要：
A pair of primers was designed according to the sequence of exon 1 of growth hormone(GH)gene published in GenBank.Single nucleotide polymorphism(SNPs)of exon 1 of GH gene was detected in Gansu modern meat sheep new breed population by polymerase chain reaction(PCR)-single strand conformation polymorphism(SSCP).The results showed that the polymorphism was detected and had two genotype(AA,AB)in Gansu modern meat sheep new breed population,which have a T/A mutation in 301 bp and G/A mutation in 305 bp of exon 1 of GH Gene.The correlation between these two genotypes with body weight was analyzed,showing that the body weight of birth,one month,two month and three month were not significant with different genotypes(P>0.05),in addition,AB are higher than AA.We concluded that GH gene possibly is major gene or linked to a major gene that effect the sheep body weight traits,and carries on the marker-assisted selection to body weight traits in sheep.

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