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当前位置: 首页 > 文章 > 外源水杨酸对马蓝叶片中蛋白水平表达的影响 应用生态学报 2010,21 (3) 689-693
Position: Home > Articles > Effects of exogenous salicylic acid on protein expression level in Baphicacanthus cusia (Nees) Bremek leaves Chinese Journal of Applied Ecology 2010,21 (3) 689-693

外源水杨酸对马蓝叶片中蛋白水平表达的影响

作  者：
向小亮;宁书菊;黄延龄;张英娇;朱仁磊;魏道智
单  位：
福建农林大学生命科学学院;福建农林大学作物科学学院
关键词：
马蓝;水杨酸;蛋白质双向电泳
摘  要：
以马蓝为试验材料,运用蛋白质双向电泳技术研究了外源水杨酸处理下马蓝叶片中蛋白质的差异表达变化.从喷施外源水杨酸处理的马蓝叶片中共获得20个显著性差异蛋白点,并成功鉴定出8个差异蛋白点,分别为ATP合成酶、α-微管蛋白、细胞分裂蛋白、甘油醛-磷酸脱氢酶和1-氨基环丙烷-1-羧酸(ACC)氧化酶.其中除乙烯合成的关键酶——ACC氧化酶表达丰度下降外,其余蛋白表达量均上升.施加外源水杨酸可以影响马蓝叶片中相关蛋白的表达水平,增强植物体的逆境抵抗力和修复能力.
译  名：
Effects of exogenous salicylic acid on protein expression level in Baphicacanthus cusia (Nees) Bremek leaves
作  者：
XIANG Xiao-liang1, NING Shu-ju2, HUANG Yan-ling1, ZHANG Ying-jiao1, ZHU Ren-lei1, WEI Dao-zhi1 (1School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2School of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China).
关键词：
Baphicacanthus cusia; salicylic acid; two-dimensional electrophoresis of protein
摘  要：
By using two-dimensional eletrophoresis method, this paper studied the protein expression level in Baphicacanthus cusia (Nees) Bremek leaves after sprayed with exogenous salicylic acid (SA). A total of significantly different 20 protein spots were obtained, among which, eight protein spots were indentified, being of ATP synthase, alpha tubulin, cell division protein, glyceraldehyde-phosphate dehydrogenase, and ACC oxidase, respectively. The expression abundance of all identified proteins was up-regulated, except for ACC oxidase which was down-regulated. Therefore, exogenous SA could affect the protein expression level in B. cusia leaves, and improve the plant resistance to environment stress and self-restoration capability.

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