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Position: Home > Articles > Development of Indirect ELISA for Clostridium chauvoei Infection in Yanbian Cattle Journal of Anhui Agricultural Sciences 2011,39 (2) 1042-1044

延边黄牛气肿疽间接ELISA诊断方法的建立

作  者:
车达;金鑫;陈莹莹;费景春;姜丹丹;吴丹;王笑玉
单  位:
吉林松原职业技术学院;吉林省动物疫病预防控制中心;延边大学农学院
关键词:
延边黄牛;气肿疽梭菌;菌体抗原;间接ELISA
摘  要:
[目的]检测延边黄牛气肿疽病原体,为牛气肿疽的检测提供科学方法和依据。[方法]采用气肿疽梭菌裂解菌体为抗原,建立检测该病原菌的酶联免疫吸附检测方法,确定其最适工作条件。[结果]气肿疽梭菌菌体裂解抗原最适包被浓度为50μg/m l;待检血清的最适稀释度为1∶200,酶标二抗的最适工作浓度为1∶2 000;对随机采集于延边地区的30份牛血清样品进行间接ELISA检测,阳性率为20%。[结论]建立的间接ELISA方法特异性和重复性好。
译  名:
Development of Indirect ELISA for Clostridium chauvoei Infection in Yanbian Cattle
作  者:
CHE Da et al (Gricultural College of Yanbian University,Yanji,Jilin 133400)
关键词:
Yanbian cattle;Clostridium chauvoei;Thallus antige;Indirect ELISA
摘  要:
[Objective] To develop an indirect ELISA for detecting Clostridium chauvoei pathogen in Yanbian cattle.[Method] Clostridium chauvoei was used as antigen,ELISA detection method was established and the optimum working conditions were also determined.[Result] The optimum concentration of Clostridium chauvoei thallus antigen for coating of plate was 50 μg/ml,the dilution of serum sample was 1∶200,the working concentration of HRP-labeled rabiit anti-bovine IgG was 1∶2 000.Moreover,all the 30 bovine serum samples collected from Yanbian canton were detected by the indirect ELISA and found the positive rate was 20%.[Conclusion] The indirect ELISA method is specific and reproducible.

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