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当前位置: 首页 > 文章 > 基于线粒体DNA D-loop序列的五华三黄鸡遗传多样性及其品种起源研究 广东农业科学 2017 (9) 106-113
Position: Home > Articles > Genetic diversity and breed origin of Wuhua Yellow chicken inferred from mitochondrial DNA D-loop sequences Guangdong Agricultural Sciences 2017 (9) 106-113

基于线粒体DNA D-loop序列的五华三黄鸡遗传多样性及其品种起源研究

作  者：
黄勋和;余哲琪;翁茁先;温金星;李威娜;钟鸣;唐亚东;钟福生
单  位：
广东客家黄畜牧有限公司;五华县田家炳中学;嘉应学院生命科学学院;广东省五华三黄鸡科技创新中心;湖南农业大学动物科学技术学院
关键词：
五华三黄鸡;线粒体DNA;D-loop;遗传多样性;系统进化
摘  要：
通过PCR产物直接测序法对五华三黄鸡保种群120个个体进行线粒体DNA(mt DNA)D-loop序列测定,研究该品种的遗传多样性和系统进化。结果显示,五华三黄鸡保留着较高水平的遗传多样性。mt DNA D-loop的C+G含量(42.5%)低于T+A含量(57.5%),核苷酸多样性和核苷酸差异均数分别为0.01298±0.00051和6.750。共检测到33个突变位点,均为转换突变,包括23个T>C转换和10个A>G转换,占分析位点的6.35%。定义了32种单倍型,其中独享型单倍型19个,单倍型多样性为0.913±0.012,遗传距离为0.013±0.003。单倍型类群主要分布在进化枝A、B、C和E,其中B为优势单倍型类群。系统进化分析和进化枝地理分布特征表明,五华三黄鸡有4个母系来源,可能受北方家鸡南迁的影响。
译  名：
Genetic diversity and breed origin of Wuhua Yellow chicken inferred from mitochondrial DNA D-loop sequences
作  者：
HUANG Xun-he;YU Zhe-qi;WENG Zhuo-xian;WEN Jin-xing;LI Wei-na;ZHONG Ming;TANG Ya-dong;ZHONG Fu-sheng;School of Life Sciences,Jiaying University;Innovation Centre for Science and Technology of Wuhua Yellow Chicken;College of Animal Science and Technology,Hunan Agricultural University;Tian Jiabing Middle School of Wuhua Country;Guangdong Ke Jia Huang Animal Husbandry Co.,Ltd.;
关键词：
Wuhua Yellow chicken;;mitochondrial DNA;;D-loop;;genetic diversity;;phylogeny
摘  要：
For depicting genetic diversity and phylogeny of Wuhua Yellow chicken,one hundred and twenty samples were used to directly sequence using PCR products of mitochondrial DNA D-loop. The results showed that the Wuhua Yellow chicken obtained high levels of genetic diversity,with nucleotide diversity,average number of nucleotide differences and haplotype diversity for 0.01298 ± 0.00051,6.750 and 0.913 ± 0.012,respectively. The content of C+G(42.5%) was lower than T+A(57.5%). A total of 33 mutation sites were detected,including 23 T to C and 10 A to G transitions,accounted for 6.35% of the analyzed sequences. Thirty-two haplotypes weredefined,with 19 exclusive haplotypes and 13 shared haloptypes. The haplotype diversity and genetic distance were 0.913±0.012 and 0.013±0.003,respectively. These haplotypes were mainly distributed in clades A,B,C and E,and B was the dominated haplogroup. Phylogenetic analysis and geographical distribution indicated that there were four maternal origins to Wuhua Yellow chicken,which was influenced to some extent,by the gene flow of domestic chicken from northern China.

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