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当前位置: 首页 > 文章 > 养殖乌鳢内脏结节病的病原分离、鉴定与药物敏感性分析 华中农业大学学报 2015 (5) 90-98
Position: Home > Articles > Isolation,identification and drug sensitivity test of causative pathogen offarmed snakehead(Channa argus)with visceral sarcoidosis Journal of Huazhong Agricultural University 2015 (5) 90-98

养殖乌鳢内脏结节病的病原分离、鉴定与药物敏感性分析

作  者：
王二龙;汪开毓;陈德芳;王均;贺扬;刘韬
单  位：
四川农业大学动物医学院;四川农业大学动物科技学院
关键词：
乌鳢;结节病;诺卡氏菌;16S rRNA;特异性引物PCR;药敏实验
摘  要：
从患内脏结节病的四川眉山地区养殖乌鳢腹水和肝、脾、肾组织中分离并纯化培养、筛选出1株致病菌,经形态结构、染色观察、生理生化实验、16SrRNA基因和特异性序列的PCR检测进行菌株的鉴定,并进行分离菌株的药物敏感性实验。结果发现,该分离株具有与诺卡氏菌Nocardia较一致的形态特征和相似的生化特性。16SrRNA基因的PCR扩增、测序和系统发育分析显示,该菌株与诺卡氏菌属的亲缘关系较近,同源性较高,与诺卡氏菌Nocardia seriolae的16SrRNA基因序列相似性高达99%以上;诺卡氏菌的特异性引物N5F1/N5R1的PCR检测结果表明该菌为诺卡氏菌;药物敏感性结果显示,该菌对四环素、强力霉素、美满霉素、阿奇霉素、磺胺异噁唑和利福平等药物敏感。
译  名：
Isolation,identification and drug sensitivity test of causative pathogen offarmed snakehead(Channa argus)with visceral sarcoidosis
作  者：
WANG Er-long;WANG Kai-yu;CHEN De-fang;WANG Jun;HE Yang;LIU Tao;College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University;College of Animal Science and Technology,Sichuan Agricultural University;
关键词：
Channa argus;;Sarcoidosis;;Nocardia seriolea;;16S rRNA;;PCR amplification;;drug sensitive test
摘  要：
A pathogenic bacterium was isolated from the ascites,liver,spleen and kidney of the diseased snakehead(Channa argus)with visceral sarcoidosis from Meishan,Sichuan Province.Morphologic structure,dyeing observation,physiological and biochemical tests,PCR amplification of 16 SrRNA gene and special sequence of the isolate were applied to determine the pathogen,and then drug sensitivity test was also carried out.The results showed that the isolate was similar to Nocardiain morphological and biochemical properties.The results of PCR amplification,sequencing and phylogenetic analysis of the 16 S rRNA gene showed that the pathogen had close genetic relationship and maximum homologies with Nocardia,having a distant sequence similarity more than 99% with N.seriolea.Moreover,the bacterium was identified as N.serioleaby PCR amplification with special primers N5F1/N5R1.Besides,the results of drug sensitive test indicated that the organism was sensitive to Tetracycline,Doxycycline,Minocycline,Azithromycin,Sulfisoxazole and Rifampicin.

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