当前位置: 首页 > 文章 > 韩国平菇菇床上流行的木霉分离物的形态学和分子生物学分析(英文) 华中农业大学学报 2004,23 (1) 157-164
Position: Home > Articles > Molecular and Morphological Analysis of Trichoderma isolates Associated with Green Mold Epidemic of Oyster Mushroom in Korea Journal of Huazhong Agricultural University 2004,23 (1) 157-164

韩国平菇菇床上流行的木霉分离物的形态学和分子生物学分析(英文)

作  者:
Park Myung Soo;Bae Kyung Sook;Yu Seung Hun
单  位:
Korea
关键词:
绿霉菌;侧耳;形态学;系统分析;分类学
摘  要:
自韩国平菇生产地分离到26株木霉(Trichoderma),基于培养特性和菌落形态,这些菌株分为4组,鉴定为Trichoderma sp.K1、Trichoderma sp.K2、T. harizianum,和T.atroviride,其中优势种为Trichoderma sp.K2,其次是Trichoderma sp.K1和T.atroviride。K1和K2不仅在形态上与其它的组有区别,而且相互在生长温度(35℃),菌落形态,分生孢子的形态,分生孢子小梗及其分枝类型,分生孢子的形态等方面有显著差异。对这些菌的ITS、EF-1(和RPB2的序列比较显示出这2个待鉴定的种(K1和K2)不仅与早期报道的木霉种有差异,而且相互间也显著不同。同时发现,EF-1(和RPB2序列比ITS序列在木霉的系统分类上更加客观可靠。基于以上研究结果,我们认为K1和K2是木霉属中的新种,即将给予它们新的名称。
译  名:
Molecular and Morphological Analysis of Trichoderma isolates Associated with Green Mold Epidemic of Oyster Mushroom in Korea
作  者:
Park Myung Soo Bae Kyung Sook Yu Seung Hun (Korea Research Institute of Bioscience and Biotechnology, Duejeon 305-600, Korea; Division of Applied Biology, Chemistry and Food Science, Chungnam National University, Daej eon 305-764, Korea)
关键词:
green mold; oyster mushroom; morphology; phylogenetic analysis; taxonomy
摘  要:
Twenty six isolates of Trichoderma were collected from oyster mushroom farms in Korea. Based on cultural and morphological characteristics, the isolates were divided into four groups and identified as Trichoderma sp. Kl-type(K1), Trichoderma sp. K2- type(K2), T. harizianum, and T. atroviride. The predominant species was K2 followed by Kl and T. atroviride. Kl and K2 were morphologically distinct not only from the other groups of Trichoderma but also from each other in the characters such as growth rate at 35℃, colony appearance, shape of conidia and phialide, and philaide branching pattern. Phylogenetic analysis of ITS, EF-1α and RPB2 sequences also revealed that the two unidentified species K1 and K2 were phylogenetically distinct not only from the previously reported Trichoderma species but also from each other. Analysis of the EF-1α and RPB2 sequences were found to be more useful for establishing systematic relationships among Thrichoderma species than that of the ITS sequence. Based on findings from this study, we proposed that Trichoderma K1 and K2 are new species. New epithets will be given to these fungi.

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