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当前位置: 首页 > 文章 > 赣南脐橙绿霉病菌对常用杀菌剂抗性监测 植物病理学报 2013,43 (5) 532-540
Position: Home > Articles > Resistance monitoring of Penicillium digitatum from Gannan navel orange to commonly used fungicides Acta Phytopathologica Sinica 2013,43 (5) 532-540

赣南脐橙绿霉病菌对常用杀菌剂抗性监测

作  者：
王明爽;孙颖;陈国庆;方贻文;夏长秀;李红叶
单  位：
浙江大学生物技术研究所;赣州市脐橙研究所
关键词：
赣南脐橙;绿霉菌;杀菌剂抗性;抗性分子机制
摘  要：
本文研究了来自赣南7个县的柑橘绿霉病菌(Penicillium digitatum)种群对该地区常用杀菌剂抑霉唑、咪鲜胺、甲基硫菌灵和百可得的抗性频率、抗性水平和对抑霉唑的抗性分子机制。结果表明:病菌对抑霉唑和咪鲜胺存在基本一致的抗性;2011和2012年病菌种群对抑霉唑和咪鲜胺的抗性频率分别为82%和90%,平均抗性倍数为51.5倍,抗性分子机制均属于IMZ-R3,即CYP51B基因启动子区发生199 bp插入的突变;病菌种群对甲基硫菌灵的抗性频率分别为82%和91%;病菌种群对百可得均表现敏感。本研究为采后柑橘病害防治药剂选择提供了科学的依据。
译  名：
Resistance monitoring of Penicillium digitatum from Gannan navel orange to commonly used fungicides
作  者：
WANG Ming-shuang;SUN Ying;CHEN Guo-qing;FANG Yi-wen;XIA Chang-xiu;LI Hong-ye;Institute of Biotechnology,Zhejiang University;Navel Orange Research Institute of Ganzhou;
关键词：
Gannan navel orange;;Penicillium digitatum;;fungicide resistance;;molecular mechanism of fungicide resistance
摘  要：
The frequency and level of resistance of Penicillium digitatum population from 7 counties of Gannan,Jiangxi Province to commonly used fungicide imazalil,prochloraz,thiophanate-methyl and iminoctadine tris,as well as the resistant molecular mechanism to imazalil were studied.The results showed that the consistent resistance to imazalil and prochloraz was presented in P.digitatum.The resistant frequencies of P.digitatum to imazalil were 82% and 90% in 2011 and 2012,respectively.The average resistant factor was 51.5.The resistance molecular mechanism of P.digitatum to imazalil from Gannan belonged to IMZ-R3,which was conferred by an insertion mutation of 199 bp in the promoter region of CYP51B gene.The resistant frequencies of P.digitatum to thiophanate-methyl were 82% and 91% in 2011 and 2012,respectively.However,all strains tested in the study were sensitive to fungicide iminoctadine tris.The study provided a scientific basis for the choice of fungicides in the post-harvest disease control of citrus.

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