Position: Home > Articles > Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants
2011,17
(1)
48-49+68
Cre/loxp重组系统在转基因植物中删除标记基因的应用
作 者：
郑雪琴
单 位：
福建师范大学生命科学学院
关键词：
Cre/loxp;转基因植物;标记基因;基因删除
摘 要：
在植物遗传转化中,标记基因是必须的,但标记基因的存在引起人们对转基因食品安全性的关注。Cre/loxp重组系统可用于删除转基因植物中的标记基因,可以通过杂交或二次转化,或瞬时表达、诱导表达、组织特异表达重组酶来获得无标记基因的转基因植株。
译 名：
Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants
作 者：
Zheng Xueqin(College of life Sciences,Fujian Normal University,Fuzhou 350108,China)
关键词：
Cre/loxp;Transgenic plant;Selectable marker gene;Gene deletion
摘 要：
Selectable marker genes are necessary for plant genetic transformation,but cause attentions about the security of genetically modified food.To remove selectable marker genes from transgenic plants by Cre/loxp recombination system,there are generally several strategies:crossing with Cre transgenic plant or constitutive expression of recombinase by re-transformation of Cre gene,or application of transient,inducible or tissue-specific expression of recombinase.
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