当前位置: 首页 > 文章 > 薄荷属种质资源遗传多样性ISSR分析 广东农业科学 2013,40 (18) 130-132,158
Position: Home > Articles > ISSR analysis on genetic diversity of Mentha germplasm resources Guangdong Agricultural Sciences 2013,40 (18) 130-132,158

薄荷属种质资源遗传多样性ISSR分析

作  者:
谢琳;王健;张玄兵;沈雁
单  位:
中国热带农业科学院椰子研究所;海南大学园艺园林学院;海南大学热带作物种质资源保护与开发利用教育部重点实验室
关键词:
薄荷属;遗传多样性;ISSR
摘  要:
利用ISSR分子标记技术对引种到海南的14个薄荷种质资源进行遗传多样性分析,从100条ISSR引物中选取12条多态性好、重复性好、条带清晰的引物,对14份样品DNA进行扩增,共得到276条条带,其中多态性条带有272条,多态性达98.32%,说明14个薄荷种质资源间存在丰富的遗传变异。遗传相似系数介于0.5181~0.8732之间,平均为0.65。利用UPGMA聚类分析,可在阈值为0.69处将其分为6大类。聚类结果与传统的形态分类法具有一致性。
译  名:
ISSR analysis on genetic diversity of Mentha germplasm resources
作  者:
XIE Lin;WANG Jian;ZHANG Xuan-bing;SHEN Yan;Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources (Hainan University),Ministry of Education;College of Horticulture and Forestry,Hainan University;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences;
关键词:
Mentha;;genetic diversity;;ISSR
摘  要:
Genetic diversity of 14 varieties of Mentha introduced to Hainan was analyzed by ISSR markers.12 primers with better polymorphism,high repeatability,clear strips were selected from 100 ISSR primers.The result showed that a total of 276 bands were detected from 14 samples of which 272 were polymorphic(98.32%),indicating that these cultivars had comparatively high genetic variation.The range of GS was 0.5181-0.8732 with an average of 0.65.And all the cultivars could be divided into 6 groups with the similarity coefficient at 0.69,by UPGMA method.The result of cluster analysis was consistent with that of traditional external appearance discrimination.

相似文章

计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊