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Position: Home > Articles > Extraction of Flavonoid from Maize Embryo with Solvent Journal of Shenyang Agricultural University 2005,36 (3)

溶剂法提取玉米胚黄酮的研究初报

作  者:
雒江菡;李秉超;于瑞洪;王蒴
单  位:
沈阳农业大学生物技术学院
关键词:
玉米胚;黄酮;提取工艺
摘  要:
采用溶剂法,研究了玉米胚中黄酮类化合物的提取,用正交实验法考察提取时间、乙醇浓度、提取温度及料液比等因素对黄酮产率的影响,用NaNO2-Al(NO3)3-NaOH比色法测定玉米胚中的总黄酮含量。结果表明:以80%的乙醇水溶液作为溶剂,固液比为11∶2,在80℃回流2h的工艺条件较好,提取率为2.46%。
译  名:
Extraction of Flavonoid from Maize Embryo with Solvent
作  者:
LUO Jiang-han,LI Bing-chao*,YU Rui-hong,WANG Shuo(College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China)
关键词:
maize embryo;flavonoid;extraction method
摘  要:
The flavonoid was separated from the maize embryo with normal experiment using alcohol to be the solvent.By orthogonal test,the effect of extraction time,ethanol concentration?extraction temperature and the ratio of feedstock to solvent on extraction was reviewed.The method of NaNO2-Al(NO3)3-NaOH was used to determines content of flavonoid.The results showed that the optimum extracting conditions are: the temperature is 80℃,the time of extraction is 2 h,the ratio of feedstock and solvent is 1∶12 and the concentration of alcohol is 80%.Under the conditions,the extraction rate is 2.46%.

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