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Position: Home > Articles > Preliminary Research on the Enzymatic Extraction Technology of Total Flavonoids from Maize Embryos Journal of Anhui Agricultural Sciences 2009,37 (31) 15417-15418+15423

酶法提取玉米胚中总黄酮工艺的初步研究

作者：

雒江菡;于瑞洪;白莉

单位：

黑龙江东方学院;黑龙江生物科技职业学院

关键词：

玉米胚;纤维素酶;黄酮;提取

摘要：

[目的]为优化天然黄酮类物质的提取条件提供一些有益参数。[方法]通过正交试验和方差分析确定了酶提取工艺的最佳参数并将其与常规有机溶剂法进行了比较。[结果]单因素试验表明,当纤维素酶浓度为0.4 mg/m l时,酶解效率最高;pH=4.5时,总黄酮的得率最高;酶解温度为50～55℃时,总黄酮的得率较高;酶解2.5 h的总黄酮得率最高。各因素对总黄酮得率的影响大小依次为:pH值>给酶量>酶解时间>酶解温度。通过正交试验和方差分析确定了玉米胚中总黄酮的提取参数为:纤维素酶浓度为0.5 mg/m l,pH=4.5,在50℃下酶解3 h,然后在80℃下浸提120 m in。酶法与有机溶剂法提取总黄酮的平均得率分别为4.65%和2.46%。[结论]酶法提取的提取条件温和,适用于工业生产,完全可以取代常规有机溶剂法。

译名：

Preliminary Research on the Enzymatic Extraction Technology of Total Flavonoids from Maize Embryos

作者：

LUO Jiang-han et al (Heilongjiang Oriental College,Harbin,Heilongjiang 150086)

关键词：

Maize embryos;Cellulase;Flavonoids;Extraction

摘要：

[Objective] The research aimed to supply some useful parameters for optimizing the extraction conditions of natural flavonoids.[Method] The optimum parameters of enzymatic extraction technology were ascertained through orthogonal test and variance analysis and it was compared with conventional organic solvent method.[Result] It was shown in single factor experiments that when the cellulase concn.was 0.4 mg/ml,the hydrolysis efficiency was highest;when the pH value was 4.5,the yield rate of total flavonoids was highest;when the hydrolysis temperature was 50～55 ℃,the yield rate of total flavonoids was relatively higher and that of total flavonoids from hydrolysis for 2.5 h was highest.The influences of various factors on the yield rate of total flavonoids were as follows: pH value > enzyme dosage > hydrolysis time > hydrolysis temperature.Through orthogonal test and variance analysis,the extraction parameters of total flavonoids from maize embryos were ascertained as hydrolysis with cellulase concn.of 0.5 mg/ml and pH value of 4.5 under 50 ℃ for 3 h,then extraction for 120 min under 80 ℃.The average yield rates of total flavonoids extracted by enzymatic method and organic solvent method were 4.65% and 2.46% resp.[Conclusion] The extraction conditions of enzymatic extraction were mild and it was suitable for industrial production and could replace conventional organic solvent method completely.

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酶法提取玉米胚中总黄酮工艺的初步研究

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