当前位置: 首页 > 文章 > 正交试验优化黑玉米籽粒中花色苷的提取 食品科学 2013,34 (12) 36-39
Position: Home > Articles > Optimization of Extraction Conditions for Anthocyanin Anthocyanins from Dark Maize FOOD SCIENCE 2013,34 (12) 36-39

正交试验优化黑玉米籽粒中花色苷的提取

作  者:
王旭;覃鸿妮;袁文娟;王久光;王国强;刘志斋;蔡一林
单  位:
西南大学玉米研究所
关键词:
黑玉米;花色苷得率;花色苷纯度;提取条件;优化
摘  要:
以西南大学新创的全黑玉米自交系SDM(super dark maize)为材料,以提高花色苷得率和纯度为目的,采用正交试验研究乙醇体积分数、固液比、提取液pH值、提取时间、提取温度5个提取条件对籽粒中花色苷得率和纯度的影响。结果表明:在90%的乙醇、固液比1:15(g/mL)、pH1、80℃保温15h的条件下,花色苷得率最高,但纯度最低,此条件下的纯度衡量指标A550nm/A275nm为0.10,得率为18.71%;在保证花色苷纯度较高的前提下,考虑最高得率,最优提取条件为:90%的乙醇、固液比1:15、pH1.5、70℃保温15h。
译  名:
Optimization of Extraction Conditions for Anthocyanin Anthocyanins from Dark Maize
作  者:
WANG Xu1,QIN Hong-ni1,2,YUAN Wen-juan1,WANG Jiu-guang1,WANG Guo-qiang1,LIU Zhi-zhai1,CAI Yi-lin1,*(1.Engineering Research Center of South Upland Agriculture,Ministry of Education,Maize Research Institute,Southwest University,Chongqing 400715,China;2.Suzhou Industrial Park Institute of Services Outsourcing,Suzhou 215123,China)
关键词:
dark maize;anthocyanin yield;anthocyanin purity;extraction condition;optimization
摘  要:
An orthogonal array design was applied to establish the optimum conditions of ethanol concentration,solidto-liquid ratio,solvent pH,temperature and time for increased yield and purity of anthocyanins from grains of Super Dark Maize(SDM).The results showed that maximum yield(18.71%) but minimum purity(A550nm/A275nm = 0.10) of anthocyanins were observed under the conditions of 90%,1:15(g/mL),1,80 ℃ and 15 h for ethanol concentration,solid-to-liquid ratio,pH,temperature and and time,respectively.The optimum extraction conditions to achieve maximum yield while increasing the purity of anthocyanins were 90% ethanol acidified to pH 1.5 as the extraction solvent with a solid-to-liquid ratio of 1:15,70 ℃ and 15 h.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊