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Position: Home > Articles > Isolation and Identification of a Halotolerant—Vibrio sp.K1-L Biotechnology Bulletin 2014,0 (4) 127-131

一株耐盐弧菌Vibrio sp.K1-L的分离与鉴定

作  者:
王菲;刘抗;范家同;成守亮;王子元
单  位:
江苏师范大学生命科学学院
关键词:
运城盐湖;弧菌属;耐盐菌;系统发育分析
摘  要:
从山西运城盐湖水样中分离出一株细菌K1-L,能在2%-10%的MGM固体培养基上出现群游现象。该菌株为革兰氏阴性菌,周生鞭毛,电镜下大小约为0.4μm×(1-2)μm,其菌落形态为圆形,灰色。菌株K1-L的最适生长盐浓度、温度和pH值的范围分别为2%-6%、30-40℃和6.0-7.0,GC含量为47.06%。通过对其形态学、生理生化试验,16S rRNA基因序列及系统发育学的分析,发现其与Vibrio diabolicus HE800T和Vibrio rotiferianus LMG 21460T等细菌的16S rRNA有高度同源性,最大的相似性均为99%,确定该菌株K1-L为弧菌属(Vibrio)。
译  名:
Isolation and Identification of a Halotolerant—Vibrio sp.K1-L
作  者:
Wang Fei;Liu Kang;Fan Jiatong;Cheng Shouliang;Wang Ziyuan;School of Life Science,Jiangsu Normal University;
关键词:
Yuncheng salt lake Vibrio Halotolerant Phylogenetic analysis
摘  要:
Strain K1-L, isolated from Yuncheng Salt Lake, Shanxi Province, could swim on the 2%-10% MGM medium. The strain K1-L was Gram-negative with polar flagellum and was 0.4 μm ×(1-2)μm under the scanning electron microscope. Strain K1-L appeared grey, rounded colony. Its optimum salt concentration, temperature and pH ranges were 2%-6%, 30-40℃ and 6.0-8.0. The GC content is 47.06%. After comparing the traditional characteristics of morphology, physiological biochemistry and 16S rRNA sequence analysis, we discovered that the strain K1-L was highly homologous to the Vibrio diabolicus HE800T and Vibrio rotiferianus LMG 21460T. The maximum similarity was 99%. It was asserted that K1-L was belonged to the genus Vibrio, we proposed the name Vibrio sp. K1-L for this strain.

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