中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 不同品种赤小豆子叶SOD提取技术优化及活性研究山西农业科学 2019 (4) 681-685

Position: Home > Articles > Study on Optimization of Extraction Technology and Activity of SOD from Cotyledons of Different Red Bean Varieties Journal of Shanxi Agricultural Sciences 2019 (4) 681-685

不同品种赤小豆子叶SOD提取技术优化及活性研究

作者：

张瑞益;李强;相微微;刘寰

单位：

榆林学院陕西省陕北矿区生态修复重点实验室

关键词：

赤小豆;SOD;超声波提取

摘要：

以京农红8号赤小豆子叶为材料,进行单因素和正交试验,优化出超声波法提取赤小豆子叶超氧化物歧化酶(SOD)的最佳条件,并分析比较不同品种赤小豆子叶中SOD的活力。结果表明,赤小豆子叶SOD最佳提取工艺条件为:液固比15∶1(m L/g),超声提取功率140 W,磷酸缓冲液pH值7.0,水浴浸提温度40℃;对赤小豆子叶SOD提取率影响的大小顺序为超声功率>磷酸缓冲液pH值>液固比>水浴温度。对比8个品种的赤小豆子叶SOD活力可以得出,不同品种赤小豆子叶SOD含量有显著差异,小丰7号SOD活力最高,SOD活力值为11.17 U/g;而吉红2号SOD活力最低,活力值仅为8.97 U/g。

译名：

Study on Optimization of Extraction Technology and Activity of SOD from Cotyledons of Different Red Bean Varieties

作者：

ZHANG Ruiyi;LI Qiang;XIANG Weiwei;LIU Huan;Key Laboratory of Ecological Restoration of Mining Area in Northern Shaanxi,Yulin University;

单位：

ZHANG Ruiyi%LI Qiang%XIANG Weiwei%LIU Huan%Key Laboratory of Ecological Restoration of Mining Area in Northern Shaanxi,Yulin University

关键词：

red bean;;SOD;;ultrasonic extraction

摘要：

This paper used Jingnonghong 8 red bean cotyledons as experimental material,through single-factor experiment and orthogonal test to find the optimum conditions in the ultrasonic extraction of superoxide dismutase(SOD). Meanwhile, the activities of SOD in different varieties of red bean were compared and analyzed. The results showed that the optimum extraction conditions of SOD from red bean cotyledons were as follows:liquid-solid ratio 15∶1(m L/g), ultrasonic extraction power 140 W, phosphate buffer pH 7.0,and water bath extraction temperature 40 ℃. The influence of four factors on SOD extraction rate of red bean cotyledons was ultrasound power>pH value of phosphate buffer>liquid-solid ratio>water bath temperature. Comparing the SOD activity of cotyledons of eight red bean varieties, the results showed that different varieties of red beans were significantly different. The SOD activity of Xiaofeng 7 was the highest, the SOD activity value was 11.17 U/g, while Jihong 2 was the lowest, the SOD activity value was only 8.97 U/g.

相似文章

QuEChERS-超高效液相色谱-串联质谱法同时测定绿豆和赤小豆中10种农药及其代谢物残留研究 [陶波, 乔禹欣, 陈国峰, 马诚义, 马红] 东北农业大学学报 2018,49 (11) 26-34+41
赤小豆种子内部寄藏真菌的分离与鉴定 [田平芳, 葛喜珍, 张志铭] 作物杂志 2006 (2) 65-67
NaCl对2种豇豆属植物种子萌发和幼苗生长的影响 [刘一明, 冯宇, 杨虎彪, 张瑜, 李欣勇, 罗小燕, 李园园, 白昌军, 刘国道] 热带农业科学 2017 (11) 11-15+26
黑核桃—赤小豆间作模式下植物光合特性的研究 [张骁博, 李春友, 张劲松, 李春兰] 河北林果研究 2016,31 (2) 109-115
NaN3诱变对赤小豆种子萌发与幼苗抗氧化系统的影响 [刘建霞, 苏迁, 周利青, 白静, 张睿] 种子 2018,37 (7) 35-38
小豆辐射诱变效应的研究 [金文林, 吕志军] 北京农学院学报 1996,011 (1) 28-33
正交试验优化超声波辅助法提取赤小豆黄酮技术研究 [刘建霞, 王润梅, 温日宇] 陕西农业科学 2015,61 (11) 1-5
绿豆的药用 [] 新农业 2005,000 (7) 34
神曲与建曲的区别 [陈顺武] 四川畜牧兽医 1987 (3) 44-45
^60Co―r射线辐射处理后小豆农艺性状诱变参数研究 [金文林, 陈学珍] 北京农学院学报 1997,012 (1) 9-14

导出引用

计量

文章访问数: 7

HTML全文浏览量: 0

PDF下载量: 0

不同品种赤小豆子叶SOD提取技术优化及活性研究

相似文章

所属期刊

山西农业科学

推荐期刊

食品工业科技

北京农学院学报