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聚乙二醇/磷酸氢二钾双水相体系萃取蛋清溶菌酶方法的建立

作  者:
齐玉;孙波;赵晓;孙欣瑶;任静;管华
单  位:
东北农业大学食品学院
关键词:
双水相萃取;蛋清溶菌酶;磷酸氢二钾
摘  要:
通过对聚乙二醇(PEG)-K2HPO4、PEG-(NH4)2SO4、PEG-C6H5O7Na3三种双水相体系进行研究,制作相图,分析得出PEG-K2HPO4是萃取蛋清溶菌酶较为合适的双水相体系。选用分子量适中的PEG2000与K2HPO4组成双水相体系分离萃取蛋清溶菌酶。研究表明,采用PEG-K2HPO4双水蛋相分离蛋清溶菌酶能达到较高的分配系数和回收率,当萃取体系组成为25%(w/w)PEG2000-35%(w/w)K2HPO4时,蛋清溶菌酶的分配系数为416.94,双水相萃取过程回收率为99.47%。
译  名:
Establishment of Extracting Lysozyme from Egg White in Polyethylene Glycol and Dipotassium Hydrogen Phosphate Aqueous Two-phase System
作  者:
QI Yu , SUN Bo , ZHAO Xiao , SUN Xinyao , REN Jing , GUAN Hua (College of Food Science , Northeast Agricultural University , Harbin , Heilongjiang 150030)
关键词:
aqueous two-phase system ; egg white lysozyme ; dipotassium hydrogen phosphate
摘  要:
Polyethylene glycol (PEG) -K2HPO4 , PEG-(NH4)2SO4 and PEG-C6H5O7Na3 aqueous two-phase systems were studied in lysozyme extraction from egg white. The results indicated that PEG-K2HPO4 aqueous two-phase system was best. Then the lysozyme was extracted and purified by PEG 2000-K2HPO4 aqueous two-phase system , which exhibited an efficient distribution coefficient and recovery. While , the separation system composed of 25% (w/w) PEG2000-35% (w/w) K2HPO4 was found to be efficient with a distribution coefficient of 416.94 , a recovery of 99.47%.

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