当前位置: 首页 > 文章 > 梭鲈的淋巴细胞培养及其核型研究 东北农业大学学报 2012,43 (3) 104-109
Position: Home > Articles > Study of peripheral blood cell cultivetion and karyotype of Sander lucioperca Linnaeus Journal of Northeast Agricultural University 2012,43 (3) 104-109

梭鲈的淋巴细胞培养及其核型研究

作  者:
黄金善;范兆廷
单  位:
东北农业大学动物科学技术学院
关键词:
梭鲈;淋巴细胞;核型
摘  要:
采用RPMI l640全培养基对梭鲈(Sander luciopercaLinnaeus)淋巴细胞进行体外培养,经空气干燥制备染色体玻片标本并对其核型进行分析。结果表明,梭鲈染色体数目为48条,包括2条中部着丝点染色体、10条亚中部着丝点染色体、12条亚端部着丝粒染色体和24条端部着丝粒染色体,未发现异型性染色体对和随体。梭鲈核型公式为2n=48,2m+10sm+12st+24t,其染色体总臂数(NF)为60。
译  名:
Study of peripheral blood cell cultivetion and karyotype of Sander lucioperca Linnaeus
作  者:
HUANG Jinshan,FAN Zhaoting(College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China)
关键词:
Sander lucioperca Linnaeus;peripheral blood;karyotype
摘  要:
The blood cell of Sander lucioperca was cultured(Linnaeus)by the whole culture medium of RPMI 1640 in vitro.By evaluating the content of colchicines and permeating time,establish a set of mature experimental method to cultivate the blood lymphocytic cell and prepare chromosome of pike-perch.Metaphase chromosome specimens of Sander lucioperca Linnaeus were prepared from short-term culture of peripheral blood lymphocytes with air-drying technique.Experimental results indicated that the diploid chromosome number was 2n=48 and the karyotype was compose of 2m,10sm,10st,12t,NF=60.

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