中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 透骨草杀虫活性成分分离鉴定及其生物活性研究 农药学学报 2012,14 (5) 127-130
Position: Home > Articles > Isolation,structure identification and bioactivity of active ingredients from Phryma leptostachya Chinese Journal of Pesticide Science 2012,14 (5) 127-130

透骨草杀虫活性成分分离鉴定及其生物活性研究

作  者：
肖新敏;胡兆农;姬志勤;师宝君;张继文;魏少鹏;吴文君
单  位：
西北农林科技大学理学院;西北农林科技大学植物保护学院
关键词：
透骨草;杀虫活性;双氧木脂素A;双氧木脂素E
摘  要：
采用柱层析分离、高效液相色谱切分和生物活性追踪法,从透骨草Phryma leptostachya L.石油醚提取物中分离出2个具有杀虫活性的化合物(B1和B2),采用质谱、核磁共振氢谱和碳谱,并结合相关文献对其结构进行鉴定,发现其分别为双氧木脂素A和E。首次以粘虫Mythimnaseparata、槐尺蠖Semiothisa cinerearia、小菜蛾Plutella xylostella、小地老虎Agrotis ypsilon和家蝇Musca domestica为试虫,测定了这2个化合物的杀虫活性。结果表明:化合物B1和B2对3龄粘虫24 h的胃毒致死中浓度(LC50值)分别为34.9和52.8μg/mL,24 h触杀致死中量(LD50值)分别为0.09和0.26μg/头;对3龄槐尺蠖24 h的胃毒LC50值分别为66.1和1 675μg/mL,触杀LD50值分别为0.049和1.33μg/头;对家蝇成虫24 h的胃毒LC50值分别为39.2和969μg/mL,触杀LD50值分别为0.047和1.12μg/头。
译  名：
Isolation,structure identification and bioactivity of active ingredients from Phryma leptostachya
作  者：
XIAO Xinmina,HU Zhaononga,JI Zhiqina,SHI Baojuna, ZHANG Jiwenb,WEI Shaopenga,WU Wenjun*a(a.College of Plant Protection,b.College of Sciences, Northwest Agricultural and Forestry University,Yangling 712100,Shaanxi Province,China)
关键词：
Phryma leptostachya;insecticidal activity;haedoxane A;haedoxane E
摘  要：
Two compounds,haedoxane A and E were isolated from the petroleum ether extract of the roots and stems of Phryma leptostachya L.by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that haedoxane A and E had stomach toxic activity with the LC50 value of 24 h 34.9 and 52.8 μg/mL against 3rd instar larvae of Mythimna separata,against 3rd instar larvae of Semiothisa cinerearia with the LC50 value of 24 h 66.1 and 1 675 μg/mL,and against Musca domestica with the LC50 value of 24 h 39.2 and 969 μg/mL.Haedoxane A and E had topical toxic activity with the LD50 value of 0.09 and 0.26 μg/larvae against 3rd instar larvae of M.separata,against 3rd instar larvae of S.cinerearia with the LD50 value of 0.049 and 1.33 μg/larvae,and against M.domestica with the LD50 value of 24 h 0.047 and 1.12 μg/fly.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46