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Position: Home > Articles > Studies on the Function of Vitreoscilla Hemoglobin Gene in Improving Surfactin Producing Rate of Bacillus subtilis Biotechnology Bulletin 2007,0 (5) 116-119+127

透明颤菌血红蛋白基因在提高枯草芽孢杆菌产脂肽功能中的研究

作  者:
王大威;胡鸢雷;刘永建;林忠平
单  位:
北京大学;大庆石油学院
关键词:
透明颤菌血红蛋白基因;枯草芽孢杆菌;脂肽;表达
摘  要:
脂肽是一类具有特殊作用的生物表面活性剂。本实验将血红蛋白基因(vhb)置于RDR细菌启动子驱动下的质粒PSET中,构建PSET-RDR-vhb重组质粒,并通过电激作用转入脂肽代谢菌株-枯草芽孢杆菌株(bacillus subtilis)ZW-3中,转化菌株经酶切和PCR电泳检测鉴定,Southern-blot杂交显示部分转化菌株中外源基因插入基因组DNA,采用一氧化碳差异色谱法测定了血红蛋白的表达量。实验进一步对转化菌株的生长曲线、总蛋白量、过氧化氢酶活性、脂肽的产率进行了测定,结果显示,相比于原始菌株,转化菌株数据均有明显提高。
译  名:
Studies on the Function of Vitreoscilla Hemoglobin Gene in Improving Surfactin Producing Rate of Bacillus subtilis
作  者:
Wang Dawei1 Hu Yuanlei2 Liu Yongjian2 Lin Zhongping1 (1Peking University,Beijing 100871;2Daqing Petroleum Institute,Daqing 163318)
关键词:
Vitreoscilla hemoglobin gene Bacillus subtilis Surfactin Expression
摘  要:
Hemoglobin of the gram-negative bacterium Vitreoscilla can bind oxygen strongly and reduce the oxygen requirement of the bacterium.A recombinant plasmid PEST-RDR-vhb was constructed and transferred into strain ZW-3(bacillus subtilis),The results showed that the expression of vhb gene could improve cell growth and enhance the production of surfactin with 31.8% higher than that of control under different dissolved oxygen concentrations.Besides,vhb gene also increased the expression of total protein quantity and CAT.

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