单 位：
淮海工学院化学工程学院;南京林业大学化学工程学院江苏省生物质绿色燃料与化学品重点实验室;淮海工学院化学工程系
摘 要：
建立光度法测定粮食中微量铜(Ⅱ)的新方法。研究表明,pH 10.0硼酸-氯化钾-氢氧化钠缓冲介质中,铜(Ⅱ)与铬黑T发生灵敏的显色反应,生成桃红色的配合物,当显色时间20min,显色剂用量达2.5mL时为最佳条件。配合物最大吸收波长为540nm,表观摩尔吸光系数(ε)为2.99×104L/(mol.cm),铜(Ⅱ)在0～1.2μg/mL范围内遵循比尔定律。样品测定结果与原子吸收分光光度法的测定结果一致。
译 名：
Spectrophotometric Determination of Trace Copper in Food Grains with Eriochrome Black T
作 者：
Siqingaowa1,2,LIU Ying-hong1,CHEN Wen-bin1,YAO Yao3(1.Jiangsu Marine Resources Develepment and Research Institute,Lianyungang 222005,China;2.School of Chemical Engineering,Huaihai Institute of Technology,Lianyungang 222005,China;3.School of Chemical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)
关键词：
eriochrome black T(EBT);spectrophotometry;copper;food grains
摘 要：
A new spectrophotometric method was developed for the determination of trace Cu(Ⅱ) in food grains based on the fact that eriochrome black T(EBT) can form a pink color complex with Cu(Ⅱ) in the presence of H2B4O7-KCl-NaOH medium at pH 10.0.The best color development results were obtained by allowing the reaction to proceed for 20 min in the presence of 2.5 mL of the color developer.The formed complex showed maximum absorption at 540 nm and an apparent molarabsorption coefficient of 2.99 × 104 L/(mol.cm).Over the range of 0-1.2μg/mL Cu(Ⅱ) solution obeyed Beer,s law.The results obtained for Cu(II) content by this method were in agreement with those obtained by atomic absorption spectrophotometry.
