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当前位置: 首页 > 文章 > 绵羊肺炎支原体感染羊细胞因子的动态变化 畜牧与兽医 2015,47 (8) 1-4
Position: Home > Articles > Dynamic change of cytokines in sheep infected with Mycoplasma ovipneumoniae Animal Husbandry & Veterinary Medicine 2015,47 (8) 1-4

绵羊肺炎支原体感染羊细胞因子的动态变化

作  者：
郭海英;王明哲;陈冬梅;陈凯丽;张晓丽;高宝德;孙延鸣
单  位：
石河子大学动物科技学院;石河子大学生命科学学院
关键词：
绵羊肺炎支原体;盘羊杂交羊;巴什拜羊;细胞因子;ELISA
摘  要：
为检测绵羊在感染绵羊肺炎支原体(MO)前后细胞因子的含量变化情况,将6只巴什拜羊和6只盘羊杂交羊人工感染MO,在感染前后分别采集静脉血,分离血清,用ELISA方法检测IL-1β、TNF-α、IL-8、IL-10及IFN-γ含量。结果显示:两组羊在感染后IL-1β浓度均升高,第21天巴什拜羊的IL-1β含量降低,且显著低于杂交羊(P<0.05);感染后两组的TNF-α含量逐渐升高,第14~21天巴什拜羊TNF-α含量开始下降,且第14天显著低于杂交羊(P<0.05),第21天极显著低于杂交羊(P<0.01);感染后第14和21天,巴什拜羊的IL-8含量开始下降,而杂交羊则继续升高,且杂交羊IL-8含量均极显著高于巴什拜羊(P<0.01);两组羊的IL-10含量在第5天达到最高,且杂交羊极显著高于巴什拜羊(P<0.01),而第14和21天巴什拜羊显著(P<0.05)和极显著(P<0.01)高于杂交羊;感染后两组羊的IFN-γ含量逐渐升高,第14~21天,巴什拜羊的IFN-γ含量开始下降,而杂交羊则继续升高,第14和21天巴什拜羊显著(P<0.05)和极显著(P<0.01)低于杂交羊。结果提示:绵羊感染MO前后细胞因子变化明显,巴什拜羊和杂交羊细胞因子变化也有差异,这对研究支原体肺炎发病机理及免疫治疗有指导意义。
译  名：
Dynamic change of cytokines in sheep infected with Mycoplasma ovipneumoniae
作  者：
GUO Haiying;WANG Mingzhe;CHEN Dongmei;CHEN Kaili;ZHANG Xiaoli;GAO Baode;SUN Yanming;College of Animal Science and Technology,Shihezi University;College of Life Science,Shihezi University;
关键词：
Mycoplasma ovipneumoniae;;Argali hybrid sheep;;Bashibai sheep;;cytokines;;ELISA
摘  要：
The aim of this study was to detect the dynamic change of serum cytokines in sheep infected with Mycoplasma Ovipneumoniae( MO). Six Bashibai sheep( BS) and 6 Argali hybrid sheep( AHS) were artificially infected with MO. The serum concentrations of IL-1β,TNF-α,IL-8,IL-10 and IFN-γ at different time points were assayed by ELISA. The results showed that IL-1β levels in two groups were elevated after infection,and the level in AHS was higher than those in BS. On the 21 st days post-infection( DPI),the level of IL-1β in BS began to decline,and significantly lower than that in AHS( P < 0. 05). TNF-α levels in two groups were increased after infection,and it reached highest on 7 DPI in BS. From 14 to 21 DPI,TNF-α level in BS declined,contrast to that in AHS,and TNF-α in AHS was higher than that in BS( P < 0. 05; P < 0. 01). On the 14 th to 21 st DPI,the level of IL-8 in BS began to decline,while the AHS was the opposite. IL-8 in AHS was higher than that in BS( P < 0. 01). The IL-10 reached the highest level on 5DPI in two groups,and IL-10 in AHS was higher than that in BS( P < 0. 01). From 14 to 21 DPI,IL-10 in AHS was lower than that in BS( P < 0. 05; P <0. 01). The contents of IFN-γ were elevated after infection in two groups. From 14 to 21 DPI,IFN-γ in AHS was higher than that in BS( P < 0. 05; P < 0. 01),and IFN-γ level declined in BS while that in AHS was elevated. It suggests that serum cytokines presented obvious changes in sheep after infection,and there exists a significant difference between two different species of sheep. This study is of guiding significance in investigating MO pathogenesis and its immune therapy.

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