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Position: Home > Articles > Use of An Acid Meter for the Rapid Determination of β-Lactamase Residue in Milk FOOD SCIENCE 2010,31 (10) 216-218

酸度计法快速检测牛奶中残留的β-内酰胺酶

作  者:
刘珊珊;周正;周巍;张子德;马俊莲
单  位:
河北农业大学食品科技学院;河北省食品质量监督检验研究院
关键词:
pH值;β-内酰胺酶;牛奶;青霉素;检测
摘  要:
为了快速、准确地检测牛奶制品中残留的β-内酰胺酶的含量,利用β-内酰胺酶分解青霉素产酸使牛奶pH值下降的原理,对人工添加了β-内酰胺酶的牛奶制品与青霉素反应后pH值的下降规律进行研究。得到牛奶中残留的β-内酰胺酶与青霉素反应的较适宜条件,从而获得快速检测牛奶制品中残留的β-内酰胺酶的方法。结果确定牛奶制品中残留的β-内酰胺酶与青霉素反应的适宜条件为温度33℃、底物质量浓度10mg/mL,检测时间仅为60min。此方法对液态纯牛奶中β-内酰胺酶的最低检出限为8.92U/mL。
译  名:
Use of An Acid Meter for the Rapid Determination of β-Lactamase Residue in Milk
作  者:
LIU Shan-shan1,ZHOU Zheng2,ZHOU Wei2,ZHANG Zi-de1,MA Jun-lian1, (1. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 2. Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050051, China)
关键词:
pH value;β-lactamase;milk;penicillin;determination
摘  要:
The principle that β-lactamase selectively decomposes β-lactam antibiotics like penicillin into acids leading to pH decline was considered to present a rapid and accurate method for the determination of β-lactamase residue in milk. β-Lactamase at a constant final concentration of 100 U/mL and penicillin were artificially added to antibiotic free milk and left to react for a certain period of time at an appropriate temperature. The optimal penicillin final concentration and reaction time and temperature were determined to be 10 mg/mL, 60 min and 33 ℃, respectively. The developed method exhibited a limit of detection of 8.92 U/mL.

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