中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 红酵母超高压诱变及其β-胡萝卜素发酵条件的初步研究 食品科学 2007,28 (9) 409-414
Position: Home > Articles > Mutation of Rhodotorula Induced by Ultra High-pressure and Its Fermentation Conditions for Carotene Production FOOD SCIENCE 2007,28 (9) 409-414

红酵母超高压诱变及其β-胡萝卜素发酵条件的初步研究

作  者：
王岁楼;陈德经;邓百万
单  位：
陕西省资源生物重点实验室
关键词：
胡萝卜素;红酵母;超高压;诱变;发酵条件
摘  要：
为了提高红酵母Rhodotorula glutinis NR06的β-胡萝卜素生产能力,利用超高压技术对其进行诱变处理,在300MPa处理10min时获得一突变株NR06-H39,其β-胡萝卜素产量达到9.64mg/L,比出发菌株NR06的6.30mg/L提高了53.02%,且遗传稳定性良好。分别研究了培养基碳、氮源等因素对该突变株胡萝卜素发酵的影响,获得了适宜的培养条件组合为培养基装量40ml/250ml、发酵液初始pH6.5、葡萄糖40g/L、(NH4)2SO45g/L、酵母膏5g/L、花生油0.4ml/L、番茄汁3ml/L和接种量8%,此时该突变株的胡萝卜素发酵产量可达12.42mg/L,比对照提高了28.84%。
译  名：
Mutation of Rhodotorula Induced by Ultra High-pressure and Its Fermentation Conditions for Carotene Production
作  者：
WANG Sui-Lou1,2,CHEN De-Jing1,DENG Bai-Wan1(1.Key Laboratory of Shaanxi Province for Resource Biology,Hanzhong 723001,China;2.Department of Food Science and Safety,China Pharmaceutical University,Nanjing 210009,China)
关键词：
carotene;Rhodotorula glutinis;ultra high-pressure;mutagenesis;fermentation conditions
摘  要：
In order to improve the ability of carotene biosynthesis,this study utilized ultra high-pressure(UHP)technology to treat Rhodotorula glutinis NR06 strain.A mutant NR06-H39 was obtained by treatment at 300 MPa for 10 min.The carotene production of this mutant reached 9.64 mg/L,an increase of 53.02% compared with 6.30 mg/L of parent strain,and its quality of genetics was proved stabile by experiments.Under the optimized fermentation conditions(glucose of 40 g/L,(NH4)2SO4 of 5 g/L,yeast extract of 5 g/L,media volume of 40 ml/250 ml shake flask,inoculation size of 8%,original pH of 6.5,peanut oil of 0.4 ml/L,and tomato extract of 3 ml/L),the β-carotene production could reach 12.42 mg/L,increasing by 22.84% compared with 9.64 mg/L of the control.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46